ORBi: Schneider Nicole - SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION

Reference : SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENAT...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Human health sciences : Multidisciplinary, general & others Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/64698

Title :	SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION
Language :	English
Author, co-author :	Schneider, Nicole [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Pôle équin, CORD, Centre Européen du Cheval de Mont le Soie > >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Lejeune, Jean-Philippe [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Deby-Dupont, Ginette P. [Université de Liège - ULg > Service d'Anesthésiologie CHU > CORD > >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Publication date :	2007
Audience :	International
Event name :	Hippos
Event date :	13./14. janvier 2007
Event organizer :	Neo-Animalia
Event place (city) :	Liège
Event country :	Belgique
Keywords :	[en] equine ; osteoarthritis ; articular chondrocytes ; synoviocytes ; free radicals ; electronic spin resonance ; anoxia ; reoxygenation
Abstract :	[en] Introduction : An oxidant activity has been implicated in the onset of equine osteoarthritis. Most of the studies have been done on articular chondrocytes, but little is known about the role of synoviocytes. Objective : Our aim was to investigate if equine articular chondrocytes, primary synoviocytes or synoviocytes of a continuous cell line are able to produce free radicals after exposure to anoxia and re-oxygenation. Methods : By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10exp7 cells; O2 consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n = 3 for each cell line). Results : Both types of synoviocytes showed a high O2 consumption, which was slower after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O2 consumption, unchanged by anoxia, and no free radical production. Conclusion : These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Research units :	Centre de l’Oxygène, Recherche et Développement - CORD
Funders :	Ministère wallon de l'agriculture, de la ruralité, de l'environnement et du tourisme
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/64698

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