Reference : SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENAT...
Scientific congresses and symposiums : Poster
Life sciences : Veterinary medicine & animal health
Human health sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/64698
SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION
English
Schneider, Nicole mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Pôle équin, CORD, Centre Européen du Cheval de Mont le Soie > >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Lejeune, Jean-Philippe mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Deby-Dupont, Ginette P. [Université de Liège - ULg > Service d'Anesthésiologie CHU > CORD > >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
2007
Yes
International
Hippos
13./14. janvier 2007
Neo-Animalia
Liège
Belgique
[en] equine ; osteoarthritis ; articular chondrocytes ; synoviocytes ; free radicals ; electronic spin resonance ; anoxia ; reoxygenation
[en] Introduction : An oxidant activity has been implicated in the onset of equine osteoarthritis. Most of the studies have been done on articular chondrocytes, but little is known about the role of synoviocytes.
Objective : Our aim was to investigate if equine articular chondrocytes, primary synoviocytes or synoviocytes of a continuous cell line are able to produce free radicals after exposure to anoxia and re-oxygenation.
Methods : By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes)
and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10exp7 cells; O2 consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated
by EPR spectroscopy with spin trapping technique (n = 3 for each cell line).
Results : Both types of synoviocytes showed a high O2 consumption, which was slower after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O2 consumption, unchanged by anoxia, and no free radical production.
Conclusion : These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Centre de l’Oxygène, Recherche et Développement - CORD
Ministère wallon de l'agriculture, de la ruralité, de l'environnement et du tourisme
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/64698

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