No full text
Poster (Scientific congresses and symposiums)
Oxidant activity of rabbit synoviocytes (HIG-82) demonstrated by oxymetry and ethylene production.
Schneider, Nicole; Lejeune, Jean-Philippe; Mouithys-Mickalad, Ange et al.
2003
 

Files


Full Text
No document available.

Send to



Details



Keywords :
synioviocytes; joint; cellular respiration; electronic paramagnetic resonance; oxymetry; HIG-82
Abstract :
[en] We are interested in a possible role of synoviocytes in the ROS production implicated in osteoarthritis, therefore we studied the response of a rabbit synoviocyte cell line (HIG-82) to variable oxygen tensions and the oxidant activity of these cells in response to stimuli. Synoviocytes were cultured at 5 and 21 % O2, their O2 consumption (cellular respiration, monitored with Clark electrode) was measured at 21% O2 and after anoxia, before and after stimulation with phorbol myristate acetate (PMA), and their oxidant response to PMA stimulation was quantified by measuring ethylene (gas chromatography) released when the substrate, alpha-keto-gamma-methylbutyric acid, is oxidised by the ROS produced by the cells. Cell growth was faster at 21 % O2 than at 5% O2, and microscopic observation revealed 2 cell populations: a few small round cells in suspension and many adherent cells. By oxymetry, we observed that a 106 synoviocytes suspension in 2 ml completely consumed O2 within 15 min, that anoxia (7 min) slightly slowed the respiration rate down and that PMA stimulation increased O2 consumption (150 % increase). The oxidant activity (ethylene production) of the cells was stimulated by PMA in a dose-dependent manner (10-9 to 10-7M) but the cell response was highly variable (from 150 to 1500 % increase) and was largely reduced by diphenyliodonium, an inhibitor of NADPH-oxidase and NO-synthase. The capacity to produce free radical species was confirmed for the small round cells by detection of an electron paramagnetic resonance (EPR) signal after stimulation. These results thus demonstrate a sensibility to O2 and an oxidant activity of synoviocytes at least related to ROS production by NADPH-oxidase activity.
Disciplines :
Veterinary medicine & animal health
Life sciences: Multidisciplinary, general & others
Author, co-author :
Schneider, Nicole ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés, Centre Européen du Cheval de Mont-le-Soie, CORD > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Lejeune, Jean-Philippe ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby-Dupont, Ginette;  Université de Liège - ULiège > CORD
Deby, Carol
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Oxidant activity of rabbit synoviocytes (HIG-82) demonstrated by oxymetry and ethylene production.
Publication date :
2003
Event place :
Ionnina, Greece
Event date :
26-29 juin 2003
Audience :
International
Funders :
Région wallonne. Ministère de l'Agriculture, de la Nature, de la Forêt, de la Ruralité, du Tourisme, du Patrimoine [BE]
Available on ORBi :
since 30 June 2010

Statistics


Number of views
171 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Bibliography


Similar publications



Contact ORBi