ORBi: Schneider Nicole - Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.

Reference : Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Multidisciplinary, general & others Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/64641

Title :	Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.
Language :	English
Author, co-author :	Schneider, Nicole [Université de Liège - ULg > CORD, Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux > >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Lejeune, Jean-Philippe [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Duyckaerts, Claire [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Sluse, Francis [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
	Deby-Dupont, Ginette [Centre de l'oxygène : Recherche et développement (C.O.R.D.) > > > >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Publication date :	2007
Journal title :	Cell Biology International
Publisher :	Academic Press
Volume :	31
Pages :	878-886
Audience :	International
ISSN :	1065-6995
City :	London
Country :	United Kingdom
Keywords :	[en] Equine ; Chondrocytes ; Oxygen ; Glucose ; Anoxia
Abstract :	[en] We investigated the oxygen (O2) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O2 and at glucose concentrations of 0, 1.0 or 4.5 g/L in the culture medium (n = 3). Afterwards, the O2 consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25 min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O2 consumption rate, which was hardly changed by anoxia. Independently from the O2 tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5 g/L glucose (n = 3). Lactate release was also independent from O2 tension, but lower for cells at 4.5 g/L glucose (n = 3). Our observations indicated that O2 consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O2 and glucose.
Research units :	Centre of Oxygen Research & Development (CORD), Institut de Chimie, B6a, Sart Tilman, University of Liège, 4000 Liège, Belgium ; Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine ; Centre Europeén du Cheval, Mont-le-Soie, Vielsam, Belgium
Funders :	Ministère de l’Agriculture et de la Ruralité de la Région Wallonne
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/64641

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