[en] Ischaemia and reperfusion are suspected to alter chondrocyte metabolism. Here, we studied the effects of three oxygen (O2) tensions on the viability of equine articular chondrocytes isolated from the cartilage of the distal interphalangeal joint of horses. Chondrocytes were cultured in alginate beads under 1%, 5% or 21% gas phase O2 concentration for 14 days, cellular growth kinetics were measured (n=6), and the cells were observed by light microscopy after staining for necrotic and apoptotic cell detection. For information about the metabolic status, the intracellular adenosine triphosphate (ATP) content was measured.
The number of chondrocytes remained stable for the first eight days, then decreased especially at 1% and 21% O2. At 21% O2, normal cells decreased and necrotic cells increased at the end of the 14 day-period. No significant variations were found at 5% O2 except for a decrease in necrotic cells at day 14. Most apoptotic cells were found at 1% O2 from days 5 to 11, and normal cells decreased during the same period. But an unexpected increase in normal cells and decrease in apoptotic cells were observed at day 14. The intracellular ATP content remained stable. It was concluded that, in a three-dimensional culture model of equine articular chondrocytes, O2 tension affected the viability of the cells after an 11-day period, with the most important effects observed at 21% and 1% O2 conditions.
Research center :
Service d’Anesthésiologie Générale et Pathologie Chirurgicale des Grands Animaux, Faculté de Médecine Vétérinaire (B 41), Université de Liège, Sart Tilman, 4000 Liège, Belgium, Centre Européen du Cheval de Mont-le-Soie, CORD.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Schneider, Nicole ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Deby, Carol
Deby-Dupont, Ginette
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Viability of equine articular chondrocytes in alginate beads exposed to different oxygen tensions.
Publication date :
2004
Journal title :
Veterinary Journal
ISSN :
1090-0233
eISSN :
1532-2971
Publisher :
Elsevier
Volume :
168
Pages :
167-173
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Ministère de l’Agriculture et de la Ruralité de la Région Wallonne
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