Reference : Induction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane D...
Scientific journals : Article
Human health sciences : Oncology
http://hdl.handle.net/2268/64589
Induction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane Diterpene
English
Block, Sébastien [Université Catholique de Louvain - UCL > > Unité d'Analyse Chimique et Physico Chimique des Médicaments > >]
Gerkens, Pascal [Université de Liège - ULG > > Histologie et Cytologie > >]
Peulen, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Jolois, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Mingeot-Leclercq, M.-P. [Université Catholique de Louvain - UCL > > Unité de Pharmacologie Cellulaire et Moléculaire > >]
Gillet, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Quetin-Leclercq, Joëlle [Université Catholique de Louvain - UCL > > Unité d’Analyse Chimique et Physico-Chimique des Médicaments > >]
Jan-2005
Anticancer Research
JG Delinassios Anticancer Research
25
1A
363-368
Yes (verified by ORBi)
International
0250-7005
Athens
Greece
[en] Diterpene ; trachylobane ; apoptosis
[en] Background: Trachylobane diterpenes are secondary metabolites quite rare in nature and their bioactivities are poorly known. Recently we have described the cytotoxic activity of ent- trachyloban-3‚-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine. Materials and Methods: Cell viability on several cell lines, cell morphology, DNA laddering, annexin V and caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis. Results: ent-trachyloban-3‚-ol exerts a dose-dependent cytotoxic effect and which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 ÌM after 24-h treatment. Conclusion: We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner.
http://hdl.handle.net/2268/64589

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