Reference : Induction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane D...

	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/64589

Title :	Induction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane Diterpene
Language :	English
Author, co-author :	Block, Sébastien [Université Catholique de Louvain - UCL > > Unité d'Analyse Chimique et Physico Chimique des Médicaments > >]
	Gerkens, Pascal [Université de Liège - ULG > > Histologie et Cytologie > >]
	Peulen, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Jolois, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Mingeot-Leclercq, M.-P. [Université Catholique de Louvain - UCL > > Unité de Pharmacologie Cellulaire et Moléculaire > >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
	Quetin-Leclercq, Joëlle [Université Catholique de Louvain - UCL > > Unité d’Analyse Chimique et Physico-Chimique des Médicaments > >]
Publication date :	Jan-2005
Journal title :	Anticancer Research
Publisher :	JG Delinassios Anticancer Research
Volume :	25
Issue/season :	1A
Pages :	363-368
Audience :	International
ISSN :	0250-7005
City :	Athens
Country :	Greece
Keywords :	[en] Diterpene ; trachylobane ; apoptosis
Abstract :	[en] Background: Trachylobane diterpenes are secondary metabolites quite rare in nature and their bioactivities are poorly known. Recently we have described the cytotoxic activity of ent- trachyloban-3‚-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine. Materials and Methods: Cell viability on several cell lines, cell morphology, DNA laddering, annexin V and caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis. Results: ent-trachyloban-3‚-ol exerts a dose-dependent cytotoxic effect and which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 ÌM after 24-h treatment. Conclusion: We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner.
Permalink :	http://hdl.handle.net/2268/64589

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