ORBi: Holvoet P. - Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of Apolipoprotein-A-I Mutants

Reference : Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of A...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/64245

Title :	Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of Apolipoprotein-A-I Mutants
Language :	English
Author, co-author :	Holvoet, P. [> > > >]
	Zhao, Za. [> > > >]
	Vanloo, B. [> > > >]
	Vos, R. [> > > >]
	Deridder, E. [> > > >]
	Dhoest, A. [> > > >]
	Taveirne, J. [> > > >]
	Brouwers, E. [> > > >]
	Demarsin, E. [> > > >]
	Engelborghs, Y. [> > > >]
	Rosseneu, M. [> > > >]
	Collen, D. [> > > >]
	Brasseur, Robert [Université de Liège > > Gembloux Agro-Bio Tech >]
Publication date :	1995
Journal title :	Biochemistry
Volume :	34
Issue/season :	41
Pages :	13334-42
ISSN :	0006-2960
Abstract :	[en] Recombinant human apolipoprotein A-I (apo A-I) and three deletion mutants: apo A-I(delta Leu44-Leu126), apo A-I(delta Glu139-Leu170), and apo A-I(delta Ala190-Gln243), purified from the periplasmic space of Escherichia coli, were studied. The rate of turbidity decrease following mixing of apo A-I(delta Ala190-Gln243) with dimyristoylphosphatidylcholine (DMPC) vesicles at 23 degrees C was 10-fold lower than that of the other apo A-I proteins, confirming that the carboxy-terminal region of apo A-I plays a role in rapid lipid binding. The Stokes radii of reconstituted high-density lipoproteins (rHDL), containing dipalmitoylphosphatidylcholine and cholesterol, were larger for the three apo A-I mutants [6.3 nm for apo A-I(delta Leu44-Leu126), 6.1 nm for apo A-I(delta Glu139-Leu170), and 6.5 nm for apo A-I(delta Ala190-Gln243)] than for intact apo A-I (5.0 nm). The mutant rHDL all contained 4 apo A-I molecules per particle as compared to 2 for intact apo A-I. Circular dichroism measurements revealed 8 alpha-helices per apo A-I molecule, 5 per apo A-I(delta Leu44-Leu126), 6 per apo A-I(delta Glu139-Leu170), and 4 per apo A-I(delta Ala190-Gln243) molecule as compared to predicted values of 8, 5, 6, and 6 alpha-helices, respectively.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/64245

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