Reference : Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of A...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/64245
Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of Apolipoprotein-A-I Mutants
English
Holvoet, P. [> > > >]
Zhao, Za. [> > > >]
Vanloo, B. [> > > >]
Vos, R. [> > > >]
Deridder, E. [> > > >]
Dhoest, A. [> > > >]
Taveirne, J. [> > > >]
Brouwers, E. [> > > >]
Demarsin, E. [> > > >]
Engelborghs, Y. [> > > >]
Rosseneu, M. [> > > >]
Collen, D. [> > > >]
Brasseur, Robert mailto [Université de Liège > > Gembloux Agro-Bio Tech >]
1995
Biochemistry
34
41
13334-42
Yes (verified by ORBi)
0006-2960
[en] Recombinant human apolipoprotein A-I (apo A-I) and three deletion mutants: apo
A-I(delta Leu44-Leu126), apo A-I(delta Glu139-Leu170), and apo A-I(delta
Ala190-Gln243), purified from the periplasmic space of Escherichia coli, were
studied. The rate of turbidity decrease following mixing of apo A-I(delta
Ala190-Gln243) with dimyristoylphosphatidylcholine (DMPC) vesicles at 23 degrees
C was 10-fold lower than that of the other apo A-I proteins, confirming that the
carboxy-terminal region of apo A-I plays a role in rapid lipid binding. The
Stokes radii of reconstituted high-density lipoproteins (rHDL), containing
dipalmitoylphosphatidylcholine and cholesterol, were larger for the three apo A-I
mutants [6.3 nm for apo A-I(delta Leu44-Leu126), 6.1 nm for apo A-I(delta
Glu139-Leu170), and 6.5 nm for apo A-I(delta Ala190-Gln243)] than for intact apo
A-I (5.0 nm). The mutant rHDL all contained 4 apo A-I molecules per particle as
compared to 2 for intact apo A-I. Circular dichroism measurements revealed 8
alpha-helices per apo A-I molecule, 5 per apo A-I(delta Leu44-Leu126), 6 per apo
A-I(delta Glu139-Leu170), and 4 per apo A-I(delta Ala190-Gln243) molecule as
compared to predicted values of 8, 5, 6, and 6 alpha-helices,
respectively.
Researchers ; Professionals
http://hdl.handle.net/2268/64245

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