Reference : Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves.
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/63960
Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves.
English
Dal Pozzo, Fabiana mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
De Clercq, Kris [Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases >]
Guyot, Hugues mailto [Université de Liège - ULg > Département clinique des animaux de production (DCP) > Médecine interne des équidés, des ruminants et des porcs >]
Vandemeulebroucke, Elise [Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases >]
Sarradin, Pierre [INRA (Nouzilly, France) > Plate-forme d’Infectiologie Experimentale >]
Vandenbussche, Frank [Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases >]
Thiry, Etienne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Saegerman, Claude mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér. >]
2009
Veterinary Microbiology
Elsevier Science
136
3-4
352-8
Yes (verified by ORBi)
International
0378-1135
Amsterdam
The Netherlands
[en] Animals ; Antibodies, Viral/blood ; Bluetongue/immunology/virology ; Bluetongue virus/genetics/growth & development/immunology ; Body Temperature/physiology ; Cattle ; Cattle Diseases/immunology/virology ; Enzyme-Linked Immunosorbent Assay/veterinary ; Female ; RNA, Viral/chemistry/genetics ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Viremia/veterinary/virology
[en] Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the manifestation of clinical signs in infected cattle. In order to study the pathogenesis of BTV-8 in this host, an animal model able to reproduce the clinical manifestations of the disease is required. In this work, two calves were subcutaneously and intravenously injected with a low passage cell-adapted strain of BTV-8. Both calves showed typical bluetongue clinical signs, including pyrexia, ocular discharge, conjunctivitis, oral mucosal congestion, development of ulcers and necrotic lesions on the lips and tongue, submandibular oedema, coronitis and oedema of the coronet and pastern region. A score was assigned depending on the severity of the lesions and a total clinical score was calculated for each animal daily and at the end of the experiment. Both calves became viraemic 24h post-infection and seroconversion occurred between 7 and 11 days P.I. In this study we present the development of a protocol of infection in calves able to reproduce the severity of the lesions observed with BTV-8 in field conditions.
http://hdl.handle.net/2268/63960
also: http://hdl.handle.net/2268/7575
10.1016/j.vetmic.2008.11.012

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