[en] Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the manifestation of clinical signs in infected cattle. In order to study the pathogenesis of BTV-8 in this host, an animal model able to reproduce the clinical manifestations of the disease is required. In this work, two calves were subcutaneously and intravenously injected with a low passage cell-adapted strain of BTV-8. Both calves showed typical bluetongue clinical signs, including pyrexia, ocular discharge, conjunctivitis, oral mucosal congestion, development of ulcers and necrotic lesions on the lips and tongue, submandibular oedema, coronitis and oedema of the coronet and pastern region. A score was assigned depending on the severity of the lesions and a total clinical score was calculated for each animal daily and at the end of the experiment. Both calves became viraemic 24h post-infection and seroconversion occurred between 7 and 11 days P.I. In this study we present the development of a protocol of infection in calves able to reproduce the severity of the lesions observed with BTV-8 in field conditions.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Dal Pozzo, Fabiana ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
De Clercq, Kris; Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases
Guyot, Hugues ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Médecine interne des équidés, des ruminants et des porcs
Vandemeulebroucke, Elise; Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases
Vandenbussche, Frank; Veterinary and Agrochemical Research Centre (Brussel) > Virology Department > Section of Epizootic Diseases
Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Language :
English
Title :
Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves.
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