Article (Scientific journals)
beta-amyloid peptide interacts specifically with the carboxy-terminal domain of human apolipoprotein E: Relevance to Alzheimer's disease
Pillot, T.; Goethals, M.; Najib, J. et al.
1999In Journal of Neurochemistry, 72 (1), p. 230-237
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Abstract :
[en] Growing evidence indicates the involvement of apolipoprotein E (apoE) in the development of late-onset and sporadic forms of Alzheimer's disease, although its exact role remains unclear. We previously demonstrated that beta-amyloid peptide (Abeta) displays membrane-destabilizing properties and that only apoE2 and E3 isoforms inhibit these properties. In this study, we clearly demonstrate that the carboxy-terminal lipid-binding domain of apoE (e.g., residues 200-299) is responsible for the Abeta-binding activity of apoE and that this interaction involves pairs of apoE amphipathic alpha-helices. We further demonstrate that Abeta is able to inhibit the association of the C-terminal domain of apoE with lipids due to the formation of Abeta/apoE complexes resistant to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. On the contrary, the amino-terminal receptor-binding domain of apoE (e.g., residues 129-169) is not able to form stable complexes with Abeta. These data extend our understanding of human apoE-dependent binding of Abeta by involving the C-terminal domain of apoE in the efficient formation of apoE/Abeta complex.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pillot, T.
Goethals, M.
Najib, J.
Labeur, C.
Lins, Laurence  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Chambaz, J.
Brasseur, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Vandekerckhove, J.
Rosseneu, M.
Language :
English
Title :
beta-amyloid peptide interacts specifically with the carboxy-terminal domain of human apolipoprotein E: Relevance to Alzheimer's disease
Publication date :
1999
Journal title :
Journal of Neurochemistry
ISSN :
0022-3042
eISSN :
1471-4159
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
72
Issue :
1
Pages :
230-237
230-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 June 2010

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