Article (Scientific journals)
Contribution Of The Hydrophobicity Gradient To The Secondary Structure And Activity Of Fusogenic Peptides
Decout, A.; Labeur, C.; Vanloo, B. et al.
1999In Molecular Membrane Biology, 16 (3), p. 237-246
Peer Reviewed verified by ORBi
 

Files


Full Text
179-cv.pdf
Publisher postprint (206.4 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Fusogenic peptides belong to a class of helical amphipathic peptides characterized by a hydrophobicity gradient along the long helical axis. According to the prevailing theory regarding the mechanism of action of fusogenic peptides, this hydrophobicity gradient causes the tilted insertion of the peptides in membranes, thus destabilizing the lipid core and, thereby, enhancing membrane fusion. To assess the role of the hydrophobicity gradient upon the fusogenic activity, two of these fusogenic peptides and several variants were synthesized. The LCAT-(57-70) peptide, which is part of the sequence of the lipolytic enzyme lecithin cholesterol acyltransferase, forms stable beta-sheets in lipids, while the apolipoprotein A-II (53-70) peptide remains predominantly helical in membranes. The variant peptides were designed through amino acid permutations, to be either parallel, perpendicular, or to retain an oblique orientation relative to the lipid-water interface. Peptide-induced vesicle fusion was monitored by lipid-mixing experiments, using fluorescent probes, the extent of peptide-lipid association, the conformation of lipid-associated peptides and their orientation in lipids, were studied by Fourier Transformed Infrared Spectroscopy. A comparison of the properties of the wild-type and variant peptides shows that the hydrophobicity gradient, which determines the orientation of helical peptides in lipids and their fusogenic activity, further influences the secondary structure and lipid binding capacity of these peptides.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Decout, A.
Labeur, C.
Vanloo, B.
Goethals, M.
Vandekerckhove, J.
Brasseur, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Rosseneu, M.
Language :
English
Title :
Contribution Of The Hydrophobicity Gradient To The Secondary Structure And Activity Of Fusogenic Peptides
Publication date :
1999
Journal title :
Molecular Membrane Biology
ISSN :
0968-7688
eISSN :
1464-5203
Publisher :
Taylor & Francis, United Kingdom
Volume :
16
Issue :
3
Pages :
237-246
237-46
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 June 2010

Statistics


Number of views
34 (3 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
13
Scopus citations®
without self-citations
9
OpenCitations
 
7

Bibliography


Similar publications



Contact ORBi