Translocation Properties Of Novel Cell Penetrating Transportan And Penetratin Analogues

[en] Novel analogues of the cell-penetrating peptides penetratin and transportan were synthesized. The distribution of the biotin-labeled peptides in Bowes melanoma cell line has been investigated by indirect fluorescence with fluorescein-streptavidin detection. The time course of uptake of (125)I-labeled transportan analogues has been characterized in the same cell line. Molecular modeling was used to analyze the penetration and the orientation of molecules in a simulated biological membrane. The results, both from molecular modeling and fluorescence studies, imply that penetratin and transportan do not enter the cells by related mechanisms and that they do not belong to the same family of translocating peptides.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Lindgren, M.

Gallet, X.

Soomets, U.

Hallbrink, M.

Brakenhielm, E.

Pooga, M.

Brasseur, Robert ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Langel, U.

Language :

English

Title :

Translocation Properties Of Novel Cell Penetrating Transportan And Penetratin Analogues

Publication date :

2000

Journal title :

Bioconjugate Chemistry

ISSN :

1043-1802

eISSN :

1520-4812

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

619-626
619-26

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 June 2010

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Bibliography

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