Article (Scientific journals)
Transferrin-Binding Protein B Of Neisseria Meningitidis: Sequence-Based Identification Of The Transferrin-Binding Site Confirmed By Site-Directed Mutagenesis
Renauld-Mongenie, G.; Lins, Laurence; Krell, T. et al.
2004In Journal of Bacteriology, 186 (3), p. 850-857
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Abstract :
[en] A sequence-based prediction method was employed to identify three ligand-binding domains in transferrin-binding protein B (TbpB) of Neisseria meningitidis strain B16B6. Site-directed mutagenesis of residues located in these domains has led to the identification of two domains, amino acids 53 to 57 and 240 to 245, which are involved in binding to human transferrin (htf). These two domains are conserved in an alignment of different TbpB sequences from N. meningitidis and Neisseria gonorrhoeae, indicating a general functional role of the domains. Western blot analysis and BIAcore and isothermal titration calorimetry experiments demonstrated that site-directed mutations in both binding domains led to a decrease or abolition of htf binding. Analysis of mutated proteins by circular dichroism did not provide any evidence for structural alterations due to the amino acid replacements. The TbpB mutant R243N was devoid of any htf-binding activity, and antibodies elicited by the mutant showed strong bactericidal activity against the homologous strain, as well as against several heterologous tbpB isotype I strains.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Renauld-Mongenie, G.
Lins, Laurence  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Krell, T.
Laffly, L.
Mignon, M.
Dupuy, M.
Delrue, Rm.
Guinet-Morlot, F.
Brasseur, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Lissolo, L.
Language :
English
Title :
Transferrin-Binding Protein B Of Neisseria Meningitidis: Sequence-Based Identification Of The Transferrin-Binding Site Confirmed By Site-Directed Mutagenesis
Publication date :
2004
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
186
Issue :
3
Pages :
850-857
850-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 June 2010

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