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| Reference : Determination Of The Minimal Fusion Peptide Of Hiv, Siv And Blv Fusion Glycoproteins |
| Scientific congresses and symposiums : Paper published in a journal | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/63501 | |||
| Determination Of The Minimal Fusion Peptide Of Hiv, Siv And Blv Fusion Glycoproteins | |
| English | |
| Lorin, A. [> > > >] | |
Charloteaux, Benoît  [Université de Liège > > Gembloux Agro-Bio Tech >] | |
| Lins, Laurence [Université de Liège > > Gembloux Agro-Bio Tech >] | |
| Stroobant, V. [> > > >] | |
| Brasseur, Robert [Université de Liège > > Gembloux Agro-Bio Tech >] | |
| 2009 | |
| Peptides For Youth - the Proceedings of the 20th American Peptidesymposium | |
| 611 | |
| 387-8 | |
| International | |
| 0065-2598 | |
| [en] The entry of enveloped viruses into target cells requires the fusion between the viral
envelope and the target cell membrane. In the case of many viruses like HIV, SIV and BLV, the fusion is mediated by class 1 fusion glycoproteins located on the viral envelope. These fusion glycoproteins contain a region at their N-terminal extremity called the “fusion peptide”, which interact with the target membrane. Many mutagenesis studies showed that this region is required for mediating membrane fusion [1]. Moreover, synthetic peptides corresponding to the fusion peptide of many glycoproteins induce membrane fusion in vitro. Despite the large number of studies on synthetic fusion peptides, the region necessary and sufficient to induce optimal membrane fusion is not known. To determine this minimal fusion peptide, we used the “tilted peptide” theory. According to this theory, a helical peptide inserting obliquely into membranes induces fusion [2]. Moreover, the more tilted the peptide is, the more important the fusion is. Then, we postulate that the minimal fusion peptide corresponds to the shortest helical fragment able to insert into the membrane with an angle close to 45°. This peptide was predicted using the IMPALA algorithm, which allow to predict peptide-membrane interactions [3]. Fusogenicity of this peptide was then assessed in liposome lipid-mixing and leakage assays and compared to the fusogenicity of smaller and longer peptides to check the validity of the prediction. This methodology was used to determine successfully the minimal fusion peptide of three viruses, HIV, SIV and BLV. | |
| Researchers ; Professionals | |
| http://hdl.handle.net/2268/63501 | |
| 10.1007/978-0-387-73657-0_169 |
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