Reference : Atomic force microscopy of supported lipid bilayers.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/63298
Atomic force microscopy of supported lipid bilayers.
English
Mingeot-Leclercq, Marie*-Paule [> > > >]
Deleu, Magali mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Brasseur, Robert mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Dufrene, Yves F [> > > >]
2008
Nature Protocols
3
10
1654-9
Yes
International
1754-2189
1750-2799
[en] Detergents ; Lipid Bilayers/chemistry ; Microscopy, Atomic Force/methods ; Surface Properties
[en] Supported lipid bilayers (SLBs) are widely used in biophysical research to investigate the properties of biological membranes and offer exciting prospects in nanobiotechnology. Atomic force microscopy (AFM) has become a well-established technique for imaging SLBs at nanometer resolution. A unique feature of AFM is its ability to monitor dynamic processes, such as the interaction of bilayers with proteins and drugs. Here, we present protocols for preparing dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers supported on mica using small unilamellar vesicles and for imaging their nanoscale interaction with the antibiotic azithromycin using AFM. The entire protocol can be completed in 10 h.
Researchers ; Professionals
http://hdl.handle.net/2268/63298
10.1038/nprot.2008.149

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