Reference : Secreted subtilisins of Microsporum canis are involved in adhesion of arthroconidia t...
Scientific congresses and symposiums : Poster
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/63260
Secreted subtilisins of Microsporum canis are involved in adhesion of arthroconidia to feline corneocytes
English
Mathy, Anne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Tabart, Jérémy [> >]
Mignon, Bernard mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Vermout, Sandy [> >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Baldo, Aline mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
2008
Yes
No
International
Imported Mycoses: Not known and not beloved
12 avril 2008
Société Belge de Mycologie Humaine et Animale
Bruxelles
Belgium
[en] dermatophyte ; adhesion ; protease
[en] Microsporum canis is a pathogenic fungus that causes a superficial skin infection called dermatophytosis mainly in cats, dogs and humans. Like other dermatophytoses, the physiopathology of this dermatosis remains largely unknown. From a fungal perspective, the infection process can be divided in three steps: adhesion of M. canis arthroconidia to corneocytes, conidial germination, and fungal invasion of the keratin network. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. However, several previously characterized secreted fungal endoproteases like subtilisins (Sub), including the keratinolytic protease Sub3, are secreted in vivo and could be involved in the first pathogenic steps. The objective of this study were (1) to develop an in vitro model to study M. canis adherence to feline corneocytes and (2) to assess whether the Sub are involved in fungal adhesion. An arthroconidial suspension was spread over the surface of reconstituted feline epidermis (RFE). Co-cultures were incubated for varying lengths of time and adherent conidia were labelled using Calcofluor white and counted. In subsequent assays arthroconidia were exposed to the serine protease inhibitor chymostatin or a mixture of two anti-Sub3 monoclonal antibodies (Mabs) one hour prior to the adherence assay. In our model, adherence of M. canis arthroconidia to RFE is time-dependent, beginning within two hours and still increasing after six hours. Chymostatin and Mabs inhibit M. canis adherence to RFE by 53 and 23 % respectively, which suggests that subtilisins and particularly Sub3, are fungal virulence factors involved in the adherence process.
grant 3.4595.04 from Fonds de la Recherche Scientifique Médicale (FRSM)
Researchers ; Professionals
http://hdl.handle.net/2268/63260

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