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VEGF-111 as a new therapeutic tool for tendon lesion
Kaux, Jean-François; Drion, Pierre; Libertiaux, Vincent et al.
2010In Osteoarthritis and Cartilage, 18 (Supplement 2), p. 22
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Keywords :
VEGF; VEGF-111; tendon
Abstract :
[en] Introduction: Tendon lesion is one of the most frequent pathology in sports and by physical workers. This pathology often becomes chronic. For this reason, it is of interest to develop new treatments. Injection of platelet-rich plasma (PRP) seems to be a promising one by releasing growth factors (GF) locally. Among all the GF released by activated platelets, the vascular endothelial growth factor-A (VEGF-A) is known to induce positive effects on vascular function and angiogenesis, and could be implicated in the healing process of tendons. Recently, a novel VEGF-A isoform was identified, the VEGF-111, a biologically active and proteolysis-resistant VEGF-A isoform, also known to present beneficial effects on ischemic diseases. This prompted us to evaluate whether VEFF-111 would have a therapeutic interest within the framework of the tendon pathology. Methods: 60 Rats were divided into 2 groups: A: control (no injection), B: VEGF-111 treatment. A 5mm defect was surgically induced in rat Achilles tendon after resection of plantaris tendon. Rats received a local injection of VEGF-111 (100ng) in situ after the surgery and were placed in their cages without immobilization. After 5, 15 and 30 days, the traumatized Achilles tendons of 10 rats of both groups were removed and dissected during their healing process. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a “Cryo-jaw”. Rats were then euthanized. Statistical analyses were made with an ANOVA. Values are significant when p-value is below 0.05. Results: Our results shown that the developed force necessary to induce tendon rupture during biomechanical tensile test was greater for tendons which had received an injection of 100ng of VEGF-111. These results were already noticed from day 5 onwards. The ratio between force and weight increased with time in both groups, but this ratio was greater for tendons which had been submitted to an injection of VEGF111. The surface area of the section of the tendons increased between 5 and 15 days followed by a stabilization. After 30 days, sections in both groups were similar. Thus, the constraint was similar after 5 and 15 days but was better for VEGF111 group after one month. Discussion - Conclusion: This experimentation has shown that a 100ng injection of VEGF-111 stimulated tendon healing process as suggested by the increased force needed to break tendons during its healing process and the increased of constraint in comparison with the control group. Other experimentations with different concentration of VEGF111 are now in process. Acknowledgement This experimentation was partially financed by “Standard de Liège 2007” and “Lejeune-Lechien 2008” grants.
Disciplines :
Laboratory medicine & medical technology
Orthopedics, rehabilitation & sports medicine
Author, co-author :
Kaux, Jean-François  ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Drion, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. - GIGA-R : Services généraux de l'Université
Libertiaux, Vincent ;  Université de Liège - ULiège > Département Argenco : Secteur MS2F > Mécanique des solides
Pascon, Frédéric ;  Université de Liège - ULiège > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur
Colige, Alain ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Le Goff, Caroline  ;  Université de Liège - ULiège > Chimie médicale
Lambert, Charles ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Gothot, André ;  Université de Liège - ULiège > Hématologie biologique et immuno hématologie
Cescotto, Serge ;  Université de Liège - ULiège > Département Argenco : Secteur MS2F > Mécanique des solides
Defraigne, Jean-Olivier ;  Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Rickert, Markus
Crielaard, Jean-Michel ;  Université de Liège - ULiège > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques - Médecine physique et réadaptation fonctionnelle
More authors (3 more) Less
Language :
English
Title :
VEGF-111 as a new therapeutic tool for tendon lesion
Publication date :
September 2010
Event name :
2010 World Congress on Osteoarthritis
Event organizer :
OsteoArthritis Research Society International (OARSI)
Event place :
Brussels, Belgium
Event date :
September 23-26, 2010
Audience :
International
Journal title :
Osteoarthritis and Cartilage
ISSN :
1063-4584
eISSN :
1522-9653
Publisher :
W.B. Saunders, London, United Kingdom
Special issue title :
Abstracts of the 2010 World Congress on Osteoarthritis
Volume :
18
Issue :
Supplement 2
Pages :
S22
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
I do not have any objections to you using this presentation, but please remember that the material is copyrighted by my medical school, and that the intellectual property is recognised as being mine. Therefore, each and every single slide will have to be acknowledged in writing on the slide itself as being mine, and the medical school logo should be displayed (you can copy and paste it from the first slide)
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since 22 June 2010

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