Reference : Platelet-Rich Plasma injection to improve tendon healing process
Scientific congresses and symposiums : Paper published in a journal
Human health sciences : Orthopedics, rehabilitation & sports medicine
http://hdl.handle.net/2268/63124
Platelet-Rich Plasma injection to improve tendon healing process
English
Kaux, Jean-François mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Drion, Pierre [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. - GIGA-R : Services généraux de l'Université >]
Renouf, Julien [> >]
Pascon, Frédéric [Université de Liège - ULg > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur >]
Libertiaux, Vincent mailto [Université de Liège - ULg > Département Argenco : Secteur MS2F > Mécanique des solides >]
Le Goff, Caroline [Université de Liège - ULg > > Chimie médicale >]
Gothot, André [Université de Liège - ULg > > Hématologie biologique et immuno hématologie >]
Cescotto, Serge [Université de Liège - ULg > Département Argenco : Secteur MS2F > Mécanique des solides >]
Defraigne, Jean-Olivier [Université de Liège - ULg > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique >]
Rickert, Markus [> >]
Crielaard, Jean-Michel [Université de Liège - ULg > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques - Médecine physique et réadaptation fonctionnelle >]
Sep-2010
Osteoarthritis and Cartilage
W.B. Saunders
18
Supplement 2
Abstracts of the 2010 World Congress on Osteoarthritis
S221
Yes (verified by ORBi)
No
International
1063-4584
London
United Kingdom
2010 World Congress on Osteoarthritis
September 23-26, 2010
OsteoArthritis Research Society International (OARSI)
Brussels
Belgium
[en] platelet-rich plasma ; PRP ; tendon
[en] Introduction
It is well known that injured tendons do not heal easily. For example, tendinopathy is a condition which often becomes chronic in the case of bad or late management. Recently, several studies, essentially in vitro and, more recently, a few in clinical practice, have demonstrated the positive effects of platelets on the healing process of different tissues. In fact, platelets contain lots of growth factors which can be released after a local injection. These growth factors have the potentiality to enhance the tendon healing process, for example after rupture or tendinopathy.
The aim of our experiment was to ascertain whether the use of Platelet-Rich Plasma (PRP) was of interest for accelerating the healing process of Achilles tendon after surgical induced lesion.

Methods
All experimental procedures and protocols used in this investigation / were reviewed and approved by the Institutional Animal Care and Use Committee of the University of Liège.
60 rats were divided into 2 groups: A: control (no injection) and B: PRP injection. A 5mm defect was surgically induced in the rats’ Achilles tendon after resection of plantaris tendon. Rats of group B received a PRP injection in situ after the surgery. Afterwards, rats of both groups were placed in their cages without immobilization.
After 5, 15 and 30 days, the traumatized Achilles tendons of 10 rats of both groups were removed and dissected during their healing process. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a “Cryo-jaw”. Rats were then euthanized.
Statistical analyses were made with an ANOVA. Values are significant when p-value is below 0.05.

Results
We observed that the force necessary to induce tendon rupture during biomechanical tensile testing increased with time in both groups; that this force was greater for tendons which had been submitted to an injection of PRP. The ratio between force and weight increased with time in both groups; that this ratio was greater for tendons which had been submitted to an injection of PRP too. There is also a significant interaction between time and the group.
The surface area of the section of the tendons increased between 5 and 15 days followed by a stabilization. After 30 days, sections in both groups were similar. Thus, the constraint was similar after 5 and 15 days but is significantly better for PRP group after one month.

Discussion – Conclusion
We demonstrated that the force necessary to induce tendon rupture during biomechanical tensile testing was greater for tendons which had been submitted to an injection of PRP. These results were observed and significant (p<0.05) from day 5 onwards. We observed too that the section of the tendon was the same in both groups after 30 days. Thus the quality of the healing tendon is better with an injection of PRP, as shown with the increase of the constraint until rupture.

Acknowledgement
This experimentation was partially financed by “Standard de Liège 2007” and “Lejeune-Lechien 2008” grants.
http://hdl.handle.net/2268/63124
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