Article (Scientific journals)
Insight into the cellular uptake mechanism of a secondary amphipathic cell penetrating peptide for siRNA delivery.
Konate, K.; Crombez, L.; Deshayes, S. et al.
2010In Biochemistry
Peer Reviewed verified by ORBi
 

Files


Full Text
290 cv.pdf
Publisher postprint (2.51 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Delivery of siRNA remains a major limitation to their clinical application and several technologies have been proposed to improve their cellular uptake. We recently described a peptide-based nanoparticle system for efficient delivery of siRNA into primary cell lines: CADY. CADY is a secondary amphipathic peptide that forms stable complexes with siRNA and improves their cellular uptake independently of the endosomal pathway. In the present work, we have combined molecular modelling, spectroscopy and membrane interaction approaches, in order to gain further insight into CADY/siRNA particle mechanism of interaction with biological membrane. We demonstrate that CADY forms stable complexes with siRNA and binds phospholipids tightly, mainly through electrostatic interactions. Binding to siRNA or phospholipids triggers a conformational transition of CADY from an unfolded state to an -helical structure, thereby stabilizing CADY/siRNA complexes and improving their interactions with cell membranes. Therefore, we propose that CADY cellular membrane interaction is driven by its structural polymorphism which enables stabilization of both electrostatic and hydrophobic contacts with surface membrane proteoglycan and phospholipids.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Konate, K.
Crombez, L.
Deshayes, S.
Decaffmeyer, M.
Thomas, Annick ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér.
Brasseur, Robert ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Aldrian, G.
Heitz, F.
Divita, G.
Language :
English
Title :
Insight into the cellular uptake mechanism of a secondary amphipathic cell penetrating peptide for siRNA delivery.
Publication date :
2010
Journal title :
Biochemistry
ISSN :
0006-2960
eISSN :
1520-4995
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 June 2010

Statistics


Number of views
87 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
72
Scopus citations®
without self-citations
54
OpenCitations
 
62

Bibliography


Similar publications



Contact ORBi