Reference : Confirmation of high cytokine clearance by hemofiltration with a cellulose triacetate me...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/6279
Confirmation of high cytokine clearance by hemofiltration with a cellulose triacetate membrane with large pores: an in vivo study
English
Delanaye, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Néphrologie >]
Lambermont, Bernard mailto [Centre Hospitalier Universitaire de Liège - CHU > > Frais communs médecine >]
Dogné, Jean-Michel [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Dubois, Bernard mailto [Centre Hospitalier Universitaire de Liège - CHU > > Néphrologie >]
Ghuysen, Alexandre mailto [Université de Liège - ULg > Département des sciences de la santé publique > Réanimation - Urgence extrahospitalière]
Janssen, Nathalie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Urgences >]
Desaive, Thomas mailto [Université de Liège - ULg > Département d'astrophys., géophysique et océanographie (AGO) > Thermodynamique des phénomènes irréversibles >]
Kolh, Philippe mailto [Université de Liège - ULg > Département des Sciences biomédicales et précliniques > Service de Biochimie et Physiologie > >]
D'Orio, Vincenzo mailto [Université de Liège - ULg > Département des sciences cliniques > Médecine d'urgence - bioch. et phys. hum. normales et path.]
Krzesinski, Jean-Marie mailto [Université de Liège - ULg > Département des sciences cliniques > Néphrologie >]
Oct-2006
International Journal of Artificial Organs
Wichtig Editore
29
10
944-948
Yes (verified by ORBi)
International
0391-3988
Milan
[en] cytokine ; endotoxin ; hemofiltration ; clearance ; septic shock
[en] Objective: To confirm in vivo the hypothesis that hemofiltration with a large pore membrane can achieve significant cytokine clearance. Method: We used a well-known animal model of endotoxinic shock (0.5 mg/kg of lipopolysaccharide from Escherichia Coli over a period of 30 mins). Six pigs were hemofiltrated for 3 hours with a large pore membrane (78 angstrom pore, 80 kDa cut off) (Sureflux FH 70, Nipro, Osaka, Japan). The ultrafiltration rate was 45 ml/kg/min. Samples were taken from arterial, venous line and in the ultrafiltrate at T120 and T240. We measured concentrations of interleukin 6, interleukin 10 and albumin. Results: At T120 and T240, the IL-6 clearances were 22 +/- 7 and 15 +/- 3 ml/min, respectively. The IL-6 sieving coefficients were 0.97 and 0.7 at T120 and T240, respectively. At T120 and T240, the IL-10 clearances were 14 +/- 4 and 10 +/- 7 ml/min, respectively. The sieving coefficients were 0.63 and 0.45 at T120 and T240, respectively. The concentrations of IL-6 and IL-10 were the same at T0 and T240. At T60 and T240, the plasmatic albumin concentrations were 24 +/- 4 g/L and 23 +/- 4 g/L, respectively ( p = 0.13). Conclusions: In this animal model of endotoxinic shock, we confirm the high cytokine clearance observed when hemofiltration is applied to a large pore membrane. The loss of albumin seems negligible. The impact of such clearances on hemodynamic stability and survival remains to be proved.
http://hdl.handle.net/2268/6279

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