Reference : Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic pr...
Scientific journals : Article
Human health sciences : Hematology
http://hdl.handle.net/2268/6263
Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
English
Feron, C. [> > > >]
Gothot, André mailto [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie biologique et immuno hématologie >]
Grosdent, Jean-Claude [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie biologique et immuno hématologie >]
Van Cauwenberge, Jean-Rémy [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Sénologie -Obstétrique CHBA >]
Schaaps, Jean-Pierre [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
Beguin, Yves [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie clinique >]
Paulus, Jean-Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
1993
Journal of Hematotherapy
2
2
259-61
Yes (verified by ORBi)
International
1061-6128
[en] Avidin ; Biotin ; Cell Differentiation ; Cell Division/drug effects ; Cell Separation/instrumentation ; Culture Media, Serum-Free ; Cytokines/pharmacology ; Fetal Blood/cytology ; Fluorescent Dyes ; Hematopoietic Stem Cells/cytology/drug effects ; Humans ; Immunosorbent Techniques ; Infant, Newborn
[en] CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells.
http://hdl.handle.net/2268/6263

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