[en] CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells.
Disciplines :
Hematology
Author, co-author :
Feron, C.
Gothot, André ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Grosdent, Jean-Claude ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Van Cauwenberge, Jean-Rémy ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Sénologie -Obstétrique CHBA
Schaaps, Jean-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Beguin, Yves ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Paulus, Jean-Michel ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Purification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
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