Reference : New use of VEGF in therapeutics: application in tendon lesions
Scientific congresses and symposiums : Paper published in a journal
Human health sciences : Orthopedics, rehabilitation & sports medicine
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/62609
New use of VEGF in therapeutics: application in tendon lesions
English
Kaux, Jean-François mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Le Goff, Caroline [Université de Liège - ULg > > Chimie médicale >]
Drion, Pierre [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. - GIGA-R : Services généraux de l'Université >]
Libertiaux, Vincent mailto [Université de Liège - ULg > Département Argenco : Secteur MS2F > Mécanique des solides >]
Pascon, Frédéric [Université de Liège - ULg > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur >]
Colige, Alain [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Lambert, Charles [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Nusgens, Betty [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Cescotto, Serge [Université de Liège - ULg > Département Argenco : Secteur MS2F > Mécanique des solides >]
Defraigne, Jean-Olivier [Université de Liège - ULg > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique >]
Rickert, Markus [ > > ]
Crielaard, Jean-Michel [Université de Liège - ULg > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques - Médecine physique et réadaptation fonctionnelle >]
Jul-2010
Clinical Chemistry
American Association for Clinical Chemistry
56
S6
Abstracts of the Scientific Posters, 2010 AACC Annual Meeting
A111
Yes (verified by ORBi)
No
International
0009-9147
1530-8561
Washington
DC
2010 AACC’s Annual Meeting
July 25-29, 2010
American Association for Clinical Chemistry
Anaheim
USA
[en] VEGF ; tendon ; rat
[en] Introduction: As demonstrated in previous studies, mechanical overload, injury and inflammation, hypoxic condition or any combination of the above could lead to increased expression of VEGF in the tendon. Thus, VEGF could participate in the healing of pathological tendons. Indeed, some authors are convinced that this neovascularization is the sign of a chronic tendinopathy while others plead in favour of it being a sign of healing processes. The VEGF111, which is a biologically active and proteolysis-resistant VEGF-A isoform, was recently identified. It is induced by ultraviolet B and genotoxic drugs. Experimentation shows that, in nude mice, tumors formed by HEK293 cells expressing VEGF111 develop a more widespread peritumoral neovascularisation than those expressing other VEGF isoforms. Good angiogenic activity and resistance to proteolysis makes VEGF111 a potential beneficial therapeutic option for ischemic diseases. The aim of our study was to determine whether if VEGF111 could have a therapeutic interest in the framework of tendinous pathology.
Methods (*): A 5mm defect was surgically induced in Achilles tendon of 60 rats. Rats were divided into 2 groups of 30: A: a control group (no injection) and B: with a VEGF111 injection. The rats of group B received an injection of 100 ng of VEGF111 in situ 1 hour after surgery on the site of the tendon lesion. Afterwards, rats of both groups were placed in their cages without immobilization. After 5, 15 and 30 days, 10 rats of each group were euthanized. The traumatized Achilles tendon of each rat was dissected and removed. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a tensile machine with “Cryo-jaw”. Statistical analyses were made with an ANOVA.
Results: A significant increase over time of the force necessary to induce tendon rupture was observed for tendons which had been submitted to an injection of VEGF111 (p=0.016). The force required to break the tendon is always greater for the VEGF111 group (p<0.05).
Discussion: We demonstrated that the force necessary to induce the rupture of a rat’s Achilles tendon during biomechanical tensile testing was greater for tendons which had been submitted to an injection of VEGF111. Thus, this experimentation showed that VEGF111 injections could accelerate the tendon healing process and increase the force needed to break tendons in their healing process.
Conclusion: VEGF111 could be a new therapy for tendon lesions. However, other experimentation using a rat model with different concentrations of VEGF111 should be made to ascertain the best concentration for this healing process.
Acknowledgement: This experimentation was partially financed by “Standard de Liège” and “Lejeune-Lechien” grants.


(*) All experimental procedures and protocols used in this investigation were reviewed and approved by the Institutional Animal Care and Use Committee of the University of Liège.
Researchers ; Professionals
http://hdl.handle.net/2268/62609
I do not have any objections to you using this presentation, but please remember that the material is copyrighted by my medical school, and that the intellectual property is recognised as being mine. Therefore, each and every single slide will have to be acknowledged in writing on the slide itself as being mine, and the medical school logo should be displayed (you can copy and paste it from the first slide).

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
New use of VEGF in therapeutics application in tendon lesions - JF KAUX.pdfAuthor postprint259.2 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.