Reference : A new combined bodian-luxol technique for staining unmyelinated axons in semithin, re...
Scientific journals : Article
Human health sciences : Neurology
http://hdl.handle.net/2268/62380
A new combined bodian-luxol technique for staining unmyelinated axons in semithin, resin-embedded peripheral nerves: a comparison with electron microscopy.
English
Deprez, Manuel [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Ceuterick-de Groote, C. [Born Bunge Foundation and University of Antwerp, > > > >]
Fumal, Arnaud mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie CHR >]
Reznik, Michel mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Martin, J. J. [Born Bunge Foundation and University of Antwerp, > > > >]
1999
Acta Neuropathologica
Springer Verlag
98
4
323-9
Yes (verified by ORBi)
International
0001-6322
1432-0533
New York
NY
[en] Axons/ultrastructure ; Cell Count ; Coloring Agents ; Humans ; Indoles ; Male ; Microscopy, Electron ; Middle Aged ; Nerve Fibers/ultrastructure ; Nerve Fibers, Myelinated/ultrastructure ; Peripheral Nerves/cytology/ultrastructure ; Resins, Plant ; Silver Staining ; Skin/innervation ; Tissue Embedding
[en] Quantitation of unmyelinated fibers (UF) in peripheral nerves has classically relied upon ultrastructural morphometry. Because this method is time-consuming, it is not typically performed in routine analysis of nerve biopsies. We applied the Bodian-Luxol technique to detect unmyelinated axons by light microscopy on semithin sections from resin-embedded nerve tissue. Estimates were compared to ultrastructural counts. The staining appeared highly specific for axons. Excellent correlation was found between optic densities and the population of UF larger than 0.5 microm. The smallest profiles detected by light microscopy had a diameter close to 0.6 microm. This new technique is not a substitute for ultrastructural quantitative morphometry of UF, as very small unmyelinated axons, especially regenerating ones, can not be reliably visualized. However, it provides a valuable light microscopic method for evaluating axonal loss among UF.
Researchers ; Professionals
http://hdl.handle.net/2268/62380
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