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| Reference : Helodermin-loaded nanoparticles: Characterization and transport across an in vitro model... |
| Scientific journals : Article | |||
| Engineering, computing & technology : Materials science & engineering Human health sciences : Pharmacy, pharmacology & toxicology Physical, chemical, mathematical & earth Sciences : Chemistry | |||
| http://hdl.handle.net/2268/6197 | |||
| Helodermin-loaded nanoparticles: Characterization and transport across an in vitro model of the follicle-associated epithelium | |
| English | |
| des Rieux, Anne [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire > >] | |
| Fievez, Virginie [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire > > >] | |
| Momtaz, Maryam [Université catholique de Louvain (UCL) > > Laboratoire de Chimie Médicinale > > >] | |
Detrembleur, Christophe  [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >] | |
| Alonso-Sande, Maria [University of Santiago de Compostela, Spain > > Department of Pharmaceutical Technology > >] | |
| Van Gelder, Jan [Lilly Development Center, Mont-Saint-Guibert, Belgium > > > >] | |
| Cauvin, Annick [Lilly Development Center, Mont-Saint-Guibert, Belgium > > > >] | |
| Schneider, Yves-Jacques [Université catholique de Louvain (UCL) > > Laboratoire de Biochimie cellulaire > > >] | |
| Preat, Véronique [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > > >] | |
| 23-Apr-2007 | |
| Journal of Controlled Release | |
| Elsevier Science Bv | |
| 118 | |
| 3 | |
| 294-302 | |
| International | |
| 0168-3659 | |
| Amsterdam | |
| [en] biomaterial ; nanomedicine ; carbon nanoparticle | |
| [en] M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of M cells on the transport of free and encapsulated helodermin - a model peptide - across the intestinal epithelium. M cells enhanced transport of intact helodermin (18-fold, Papp 3 X 10(-6) cm s(-1)). As pegylation increased nanoparticle transport by M cells, helodermin was encapsulated in 200 mu nanoparticles containing PEG-b-PLA:PLGA 1:1. Stability of the selected formulation was demonstrated in simulated gastric and intestinal fluids. M cells increased the transport of helodermin encapsulated in these nanoparticles by a factor of 415, as compared to Caco-2 cells. Transport of free and encapsulated helodermin occurred most probably by endocytosis. In conclusion, M cells improved helodermin transport across the intestinal epithelium, confirming their high potential for oral delivery of peptides. | |
| Center for Education and Research on Macromolecules (CERM) | |
| Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; The Région Wallonne (DGTRE), First Europe (n° 215099 and 415847) and VACCINOR project (WINOMAT) ; The Fond Scientifique de Recherche of the Université catholique de Louvain | |
| Researchers | |
| http://hdl.handle.net/2268/6197 | |
| 10.1016/j.jconrel.2006.12.023 | |
| http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3D-4MP566N-1-1&_cdi=4944&_user=532038&_orig=browse&_coverDate=04%2F23%2F2007&_sk=998819996&view=c&wchp=dGLbVlz-zSkzV&md5=2fce81880af5b91e71e004c995ab5128&ie=/sdarticle.pdf | |
| http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/description#description | |
| The authors acknowledge Journal of Controlled Release (Elsevier) for allowing them to archive this paper. |
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