[en] M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of M cells on the transport of free and encapsulated helodermin - a model peptide - across the intestinal epithelium. M cells enhanced transport of intact helodermin (18-fold, Papp 3 X 10(-6) cm s(-1)). As pegylation increased nanoparticle transport by M cells, helodermin was encapsulated in 200 mu nanoparticles containing PEG-b-PLA:PLGA 1:1. Stability of the selected formulation was demonstrated in simulated gastric and intestinal fluids. M cells increased the transport of helodermin encapsulated in these nanoparticles by a factor of 415, as compared to Caco-2 cells. Transport of free and encapsulated helodermin occurred most probably by endocytosis. In conclusion, M cells improved helodermin transport across the intestinal epithelium, confirming their high potential for oral delivery of peptides.
Research center :
Center for Education and Research on Macromolecules (CERM)
First Europe (n° 215099 and 415847) VACCINOR project (WINOMAT)
Funders :
BELSPO - Service Public Fédéral de Programmation Politique scientifique [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE] UCL - Université Catholique de Louvain [BE]
Commentary :
The authors acknowledge Journal of Controlled Release (Elsevier) for allowing them to archive this paper.
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