Reference : Whole genome expression profiling of the medial and lateral substantia nigra in Parkinso...
Scientific journals : Article
Human health sciences : Neurology
http://hdl.handle.net/2268/61892
Whole genome expression profiling of the medial and lateral substantia nigra in Parkinson's disease.
English
Moran, L. B. [Imperial College London > Department of Neuropathology > > >]
Duke, D. C. [Imperial College London > Department of Neuropathology > > >]
Deprez, Manuel mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Dexter, D. T. [Imperial College London > Department of Cellular and Molecular Neuroscience > > >]
Pearce, R. K. B. [Imperial College London > Department of Neuropathology > > >]
Graeber, M. B. [Imperial College London > Department of Neuropathology > > >]
2006
Neurogenetics
Springer Verlag
7
1
1-11
Yes (verified by ORBi)
International
1364-6745
1364-6753
New York
NY
[en] Aged ; Aged, 80 and over ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Genome, Human ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Oligonucleotide Array Sequence Analysis ; Parkinson Disease/genetics ; Reproducibility of Results ; Substantia Nigra/anatomy & histology/physiology
[en] We have used brain tissue from clinically well-documented and neuropathologically confirmed cases of sporadic Parkinson's disease to establish the transcriptomic expression profile of the medial and lateral substantia nigra. In addition, the superior frontal cortex was analyzed in a subset of the same cases. DNA oligonucleotide microarrays were employed, which provide whole human genome coverage. A total of 570 genes were found to be differentially regulated at a high level of significance. A large number of differentially regulated expressed sequence tags were also identified. Levels of mRNA sequences encoded by genes of key interest were validated by means of quantitative real-time polymerase chain reaction (PCR). Comparing three different normalization procedures, results based on the recently published GeneChip Robust Multi Array algorithm were found to be the most accurate predictor of real-time PCR results. Several new candidate genes which map to PARK loci are reported. In addition, the DNAJ family of chaperones is discussed in the context of Parkinson's disease pathogenesis.
UK Parkinson’s Disease Society
Researchers ; Professionals
http://hdl.handle.net/2268/61892
10.1007/s10048-005-0020-2
The original publication is available at www.springerlink.com author can archive post-print (ie final draft post-refereeing)

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