Article (Scientific journals)
Whole genome expression profiling of the medial and lateral substantia nigra in Parkinson's disease.
Moran, L. B.; Duke, D. C.; Deprez, Manuel et al.
2006In Neurogenetics, 7 (1), p. 1-11
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Keywords :
Aged; Aged, 80 and over; Female; Gene Expression Profiling; Gene Expression Regulation; Genome, Human; Humans; Male; Middle Aged; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Parkinson Disease/genetics; Reproducibility of Results; Substantia Nigra/anatomy & histology/physiology
Abstract :
[en] We have used brain tissue from clinically well-documented and neuropathologically confirmed cases of sporadic Parkinson's disease to establish the transcriptomic expression profile of the medial and lateral substantia nigra. In addition, the superior frontal cortex was analyzed in a subset of the same cases. DNA oligonucleotide microarrays were employed, which provide whole human genome coverage. A total of 570 genes were found to be differentially regulated at a high level of significance. A large number of differentially regulated expressed sequence tags were also identified. Levels of mRNA sequences encoded by genes of key interest were validated by means of quantitative real-time polymerase chain reaction (PCR). Comparing three different normalization procedures, results based on the recently published GeneChip Robust Multi Array algorithm were found to be the most accurate predictor of real-time PCR results. Several new candidate genes which map to PARK loci are reported. In addition, the DNAJ family of chaperones is discussed in the context of Parkinson's disease pathogenesis.
Disciplines :
Neurology
Author, co-author :
Moran, L. B.;  Imperial College London > Department of Neuropathology
Duke, D. C.;  Imperial College London > Department of Neuropathology
Deprez, Manuel ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Dexter, D. T.;  Imperial College London > Department of Cellular and Molecular Neuroscience
Pearce, R. K. B.;  Imperial College London > Department of Neuropathology
Graeber, M. B.;  Imperial College London > Department of Neuropathology
Language :
English
Title :
Whole genome expression profiling of the medial and lateral substantia nigra in Parkinson's disease.
Publication date :
2006
Journal title :
Neurogenetics
ISSN :
1364-6745
eISSN :
1364-6753
Publisher :
Springer Verlag, New York, United States - New York
Volume :
7
Issue :
1
Pages :
1-11
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
UK Parkinson’s Disease Society
Available on ORBi :
since 11 June 2010

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