Quantitative Analysis of N-Acetylcysteine and Its Pharmacopeial Impurities in a Pharmaceutical Formulation by Liquid Chromatography-Uv Detection-Mass Spectrometry

Toussaint, B.; Pitti, C.; Streel, B.; Ceccato, Attilio; Hubert, Philippe; Crommen, Jacques

doi:10.1016/S0021-9673(00)00741-X

Article (Scientific journals)

Quantitative Analysis of N-Acetylcysteine and Its Pharmacopeial Impurities in a Pharmaceutical Formulation by Liquid Chromatography-Uv Detection-Mass Spectrometry

Toussaint, B.; Pitti, C.; Streel, B. et al.

2000 • In Journal of Chromatography. A, 896 (1-2), p. 191-9

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/6185

DOI
10.1016/S0021-9673(00)00741-X

PubMed
11093654

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Abstract :

[en] A new method for the simultaneous determination of N-acetylcysteine and its pharmacopeial impurities, cysteine, cystine, N,N'-diacetylcystine and N,S-diacetylcysteine in an effervescent tablet has been developed. The method is based on on-line LC-UV-MS using a pneumatically-assisted electrospray interface (ionspray). The stability of the thiol moieties of the analytes was ensured by the acidic pH of the LC mobile phase. Quantitation of N-acetylcysteine was performed with UV detection to avoid ion-source overloading effect due to its higher concentration, whereas the impurities could be easily separated and quantified in MS. The method was validated in terms of stability, linearity, precision and accuracy.

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Toussaint, B.

Pitti, C.

Streel, B.

Ceccato, Attilio ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Hubert, Philippe ; Université de Liège - ULiège > Chimie analytique

Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Language :

English

Title :

Quantitative Analysis of N-Acetylcysteine and Its Pharmacopeial Impurities in a Pharmaceutical Formulation by Liquid Chromatography-Uv Detection-Mass Spectrometry

Publication date :

27 October 2000

Journal title :

Journal of Chromatography. A

ISSN :

0021-9673

eISSN :

1873-3778

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

896

Issue :

1-2

Pages :

191-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 February 2009

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