Article (Scientific journals)
DNA immunisation. New histochemical and morphometric data.
Ehirchiou, D.; Zorzi, Willy; Biemans, R. et al.
2002In European Journal of Histochemistry, 46 (3), p. 215-22
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Keywords :
Animals; Antibodies, Monoclonal/diagnostic use; Antigens, CD3/immunology; Antigens, CD8/immunology; Antigens, Protozoan; B-Lymphocytes/immunology; Biotin/diagnostic use; Carbon; Coloring Agents; DNA/immunology; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Germinal Center/ultrastructure; Immunohistochemistry/methods; Injections, Intramuscular; Mice; Mice, Inbred BALB C; Plasmids/genetics; Protozoan Proteins/metabolism; T-Lymphocytes/immunology
Abstract :
[en] Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the SAG1 protein, a membrane antigen of Toxoplasma gondii. Specific anti-SAG1 antibodies were detected on days 16 and 36 after injection of coding plasmids. The results of ELISAs showed that the SAG1-specific antibodies are of the IgG2a class. Morphometric analyses were done on serial immunostained cryosections of spleen and draining or non-draining lymph nodes. This new approach made it possible to evaluate the chronological changes induced by DNA immunisation in the germinal centres (in number and in size). Significant increases in the number of germinal centres were measured in the spleen and only in draining lymph nodes after plasmid injection, the measured changes of the germinal centers appeared to result from the adjuvant stimulatory effect of the plasmidic DNA since both the coding and the noncoding plasmid DNA induced them. No measurable changes were recorded in the T-dependent zone of lymph organs.
Disciplines :
Immunology & infectious disease
Author, co-author :
Ehirchiou, D.
Zorzi, Willy ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques
Biemans, R.
Vanderbeeke, A.
Haumont, M.
Collin, S.
Jolois, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Bollen, A.
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ;  Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Language :
English
Title :
DNA immunisation. New histochemical and morphometric data.
Publication date :
2002
Journal title :
European Journal of Histochemistry
ISSN :
1121-760X
eISSN :
2038-8306
Publisher :
Luigi Ponzio E Figlio Editori, Pavia, Italy
Volume :
46
Issue :
3
Pages :
215-22
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 June 2010

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