[en] It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-beta1 was up-regulated in the dermis of old donors as well as the TGF-beta1-regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione S-Transferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Borlon, Celine; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Dept of Biology, Faculty of Sciences > Research Unit on Cellular Biology
Weemaels, Geoffroy; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Dept of Biology, Faculty of Sciences > Research Unit on Cellular Biology
Godard, Patrice; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Dept of Biology, Faculty of Sciences > Research Unit on Cellular Biology
Debacq-Chainiaux, Florence; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Dept of Biology, Faculty of Sciences > Research Unit on Cellular Biology
Lemaire, Pierre; Cente Hospitalier Regional de Namur > Geriatric Unit
Deroanne, Christophe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Toussaint, Olivier; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Dept of Biology, Faculty of Sciences > Research Unit on Cellular Biology
Language :
English
Title :
Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study.
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