Reference : Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement as...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/612
Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders
English
Monchaud, David [Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France > > > >]
Allain, C. [Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France > > > >]
Bertrand, Hélène [Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France > > > >]
Smargiasso, Nicolas mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie physique, spectrométrie de masse >]
Rosu, Frédéric [Université de Liège - ULg > Département de chimie (sciences) > Chimie physique, spectrométrie de masse >]
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie physique, spectrométrie de masse >]
De Cian, Anne [INSERM, U565, Acides Nucle´iques: Dynamique, Ciblage et Fonctions Biologiques, Muse´um National d’histoire Naturelle USM 503, 43, Rue Cuvier, 75005 Paris, France > > > >]
Mergny, Jean-Louis [INSERM, U565, Acides Nucléques: Dynamique, Ciblage et Fonctions Biologiques, Musém National d’histoire Naturelle USM 503, 43, Rue Cuvier, 75005 Paris, France > > > >]
Teulade-Fichou, Marie-Paule [Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France > > > >]
2008
Biochimie
Elsevier
90
8
Special issue: Targeting DNA, part II
1207-1223
Yes (verified by ORBi)
International
0300-9084
Paris
France
[en] G-quadruplex ; thiazole orange ; fluorescence ; noncovalent ; mass spectrometry ; DNA
[en] We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex and duplex-DNA matrices. The original protocol was tested using various quadruplex and
duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metalloorganic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Researchers
http://hdl.handle.net/2268/612
10.1016/j.biochi.2008.02.019
The final version of this article has been published by Elsevier as: D. Monchaud, C. Allain, H. Bertrand, N. Smargiasso, F. Rosu, V. Gabelica, A. De Cian, J.-L. Mergny, and M.-P. Teulade-Fichou, "Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders", Biochimie (2008), 90(8), Special issue "Targeting DNA Part II", 1207-1223

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