G-quadruplex; thiazole orange; fluorescence; noncovalent; mass spectrometry; DNA
Abstract :
[en] We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex and duplex-DNA matrices. The original protocol was tested using various quadruplex and
duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metalloorganic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.
Research center :
Giga-Systems Biology and Chemical Biology - ULiège
Monchaud, David; Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France
Allain, C.; Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France
Bertrand, Hélène; Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France
Smargiasso, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Rosu, Frédéric ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
De Cian, Anne; INSERM, U565, Acides Nucle´iques: Dynamique, Ciblage et Fonctions Biologiques, Muse´um National d’histoire Naturelle USM 503, 43, Rue Cuvier, 75005 Paris, France
Mergny, Jean-Louis; INSERM, U565, Acides Nucléques: Dynamique, Ciblage et Fonctions Biologiques, Musém National d’histoire Naturelle USM 503, 43, Rue Cuvier, 75005 Paris, France
Teulade-Fichou, Marie-Paule; Institut Curie, Section Recherche, CNRS UMR176, Centre Universitaire Paris XI, Bat. 110, 91405 Orsay, France
Language :
English
Title :
Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders
Publication date :
2008
Journal title :
Biochimie
ISSN :
0300-9084
eISSN :
1638-6183
Publisher :
Elsevier, Paris, France
Special issue title :
Special issue: Targeting DNA, part II
Volume :
90
Issue :
8
Pages :
1207-1223
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Commentary :
The final version of this article has been published by Elsevier as: D. Monchaud, C. Allain, H. Bertrand, N. Smargiasso, F. Rosu, V. Gabelica, A. De Cian, J.-L. Mergny, and M.-P. Teulade-Fichou, "Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders", Biochimie (2008), 90(8), Special issue "Targeting DNA Part II", 1207-1223
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