Article (Scientific journals)
Fibroblasts Induce the Assembly of the Macromolecules of the Basement Membrane
Delvoye, Pierre; Piérard, D.; Noël, Agnès et al.
1988In Journal of Investigative Dermatology, 90 (3), p. 276-82
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Abstract :
[en] The mechanism regulating the deposition of basement membrane components (BMCs) in a polymeric structure at the junction with the connective tissues is not yet understood. Cultures and cocultures of epithelial BMC-producing cells (L2 or PER cells) and fibroblasts were prepared in several experimental conditions and the organization of BMCs was studied by immunofluorescence. The pattern of BMCs in pure cultures of L2 or pulmonary epithelial rat (PER) cells consisted of intra- and extracellular granular deposits. At very high density, the cell contours were also underlined by a disrupted network of BMC deposits. A different fibrillar plexus--containing laminin, collagen type IV, and heparan-sulfate proteoglycan resistant to deoxycholate treatment and distant from the cell membrane--was observed in cocultures of L2 or PER cells with fibroblasts. Fibrils of fibronectin and/or collagen type I were most often dissociated from this plexus of BMCs. Similar results were obtained by adding a conditioned medium of L2 or PER cells to confluent fibroblasts, even when the cells were killed. Pure laminin also bound to the fibroblast layer. A coated film of fibronectin or polymeric collagen type I was unable to bind BMC provided by a conditioned medium. It is suggested that molecule(s) synthesized by fibroblasts and deposited in the pericellular matrix are involved in the assembly of BMCs.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Delvoye, Pierre ;  Université de Liège - ULiège > Dermatologie
Piérard, D.
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Lapiere, C. M.
Language :
English
Title :
Fibroblasts Induce the Assembly of the Macromolecules of the Basement Membrane
Publication date :
March 1988
Journal title :
Journal of Investigative Dermatology
ISSN :
0022-202X
eISSN :
1523-1747
Publisher :
Nature Publishing Group, New York, United States - New York
Volume :
90
Issue :
3
Pages :
276-82
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 June 2010

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