Reference : Development of a strategy to study toxicodynamic of pollutants in spawning sea turtle...
Scientific congresses and symposiums : Poster
Life sciences : Zoology
Development of a strategy to study toxicodynamic of pollutants in spawning sea turtles from the French West Indies
Dyc, Christelle[Université de Liège - ULg > Département des sciences et gestion de l'environnement > Océanologie >]
Bouquegneau, Jean-Marie[Université de Liège - ULg > Département des sciences et gestion de l'environnement > Océanologie >]
De Pauw-Gillet, Marie-Claire[Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Thomé, Jean-Pierre[Université de Liège - ULg > Département des sciences et gestion de l'environnement > Ecologie animale et écotoxicologie >]
Das, Krishna[Université de Liège - ULg > Département des sciences et gestion de l'environnement > Océanologie >]
Cayol, Claire[Office National de la Chasse et de la Faune Sauvage (ONCFS), Martinique, French West Indies > > > Veterinary and Sea Turtles Official Representative >]
Delcroix, Eric[Association Kap 'Natirel > Guadeloupe, French West Indies > > Guadeloupe Sea Turtle Conservation Project Coordinator >]
88.1 cm x 118.9 cm
15th Benelux Congress of Zoology
30 - 31 October 2008
University of Liege
[en] Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant lack of data dealing with toxicants such as metals and persistent organic pollutants (POPs) in sea turtles. We developed a strategy to apprehend levels, effects and transfer to offspring of several pollutants in sea turtles. Sampling of blood, subcutaneous tissue and eggs of 15 gravid C. mydas and E. imbricata was carried out between July and September 2008 in Martinique (Diamant’s beach) and Guadeloupe (Petite Terre and Marie-Galante). Blood was collected from the dorso-cervical sinus and subcutaneous tissue was sampled in shoulder of the spawning females using a 5 mm biopsy punch (Kai Europe GmbH, Germany). Total blood and serum were successfully taken for metal, POP and biomarker investigations. T-mercury was analyzed by DMA milestones while PCBs, DDT and chlordecone were analyzed by EDC Ni63 high performance gas chromatography HPLC. Samples of serum were analyzed for vitamins (A and E) by HPLC and for thyroid hormones (triiodothyronine and thyroxine) by radioimmunoassay. In parallel to this field study, a cell model using 3T3-L1 cell line was built up to test in vitro effects of PCBs and mercury as well as the relationship between in vitro exposure and fat mobilization. Preliminary results showed a dose-response relationship between increased Aroclor 1234 and 1252 concentrations (0.5 ppb, 1 ppb and 1.5 ppb) and adipocyte mortality (Nucleocounter).
The strategy we propose here will bring further insights on levels and potential impact of pollutants on female sea turtles and their offspring.
Toxicodynamic of pollutants in poikilotherm species (turtles)
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