Article (Scientific journals)
A major quantitative trait locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase
Courbot, M.; Willems, G.; Motte, Patrick et al.
2007In Plant Physiology, 144 (2), p. 1052-1065
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Abstract :
[en] Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri sp. halleri. Therefore, an interspecific cross was made between A. halleri and its closest nontolerant interfertile relative, Arabidopsis lyrata sp. petraea, and a first-generation backcross population (BC1) was used to map quantitative trait loci (QTL) for Cd tolerance. Three QTL were identified, which explained 43%, 24%, and 16% of the phenotypic variation in the mapping population. Heavy metal transporting ATPases4 (HMA4), encoding a predicted heavy metal ATPase, colocalized with the peak of the major QTL Cdtol-1 and was consequently further studied. HMA4 transcripts levels were higher in the roots and the shoots of A. halleri than in A. lyrata sp. petraea. Furthermore, HMA4 was also more highly expressed in all BC1 genotypes harboring the HMA4 A. halleri allele at the QTL Cdtol-1, independently of the presence of an A. halleri allele at the two other QTL. Overexpression of AhHMA4 in yeast (Saccharomyces cerevisiae) supported a role of HMA4 in zinc (Zn) and Cd transport by reducing the Cd and Zn contents of the yeast cells. In epidermal tobacco (Nicotiana tabacum) cells, AhHMA4:green fluorescent protein was clearly localized in the plasma membrane. Taken together, all available data point to the elevated expression of HMA4P(1B)-type ATPase as an efficient mechanism for improving Cd/ Zn tolerance in plants under conditions of Cd/ Zn excess by maintaining low cellular Cd2+ and Zn2+ concentrations in the cytoplasm.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Courbot, M.
Willems, G.
Motte, Patrick ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Arvidsson, S.
Roosens, N.
Saumitou-Laprade, P.
Verbruggen, N.
Language :
English
Title :
A major quantitative trait locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase
Publication date :
June 2007
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
Amer Soc Plant Biologists, Rockville, United States - Maryland
Volume :
144
Issue :
2
Pages :
1052-1065
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 June 2010

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