ORBi: MELIN Pierrette - Evaluation of the group B differential agar for the detection of group B streptococci from vaginal specimens

Reference : Evaluation of the group B differential agar for the detection of group B streptococci fr...


	Document type :	Scientific congresses and symposiums : Paper published in a book
	Discipline(s) :	Human health sciences : Immunology & infectious disease Human health sciences : Laboratory medicine & medical technology
	To cite this reference:	http://hdl.handle.net/2268/60849

Title :	Evaluation of the group B differential agar for the detection of group B streptococci from vaginal specimens
Language :	English
Author, co-author :	MELIN, Pierrette [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
	Rodriguez Cuns, Grisel [Universitad de la Republica, Montevideo, Uruguay > > > >]
	Lorquet, Sophie [ > > ]
	HAYETTE, Marie-Pierre [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
	CHRISTIAENS, Geneviève [Centre Hospitalier Universitaire de Liège - CHU > > Direction médicale >]
	Foidart, J. M. [Université de Liège - ULg > Gynécologie Obstétrique > > >]
	De Mol, Patrick [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale >]
Publication date :	May-2004
Main document title :	Program and Abstracts of the 104th General Meeting of the American Society for Microbiology
Editor :	American Society of Microbiology
Publisher :	American Society for Microbiology (ASM)
Pages :	Abstract du poster C-027
On invitation :	No
Audience :	International
City :	Washington
Country :	USA
Event name :	104th General Meeting of the American Society for Microbiology
Event date :	Du 23 au 27 mai 2004
Event organizer :	American Society of Microbiology
Event place (city) :	New Orleans
Event country :	USA
Keywords :	[en] Group B streptococci ; Differential agar ; vaginal specimen
Abstract :	[en] Background Group B streptococci (GBS) are the leading cause of severe perinatal infections. Most current guidelines for the prevention of GBS perinatal disease are based on prenatal screening culture for vaginal GBS colonisation. Use of selective and differential media could improve the sensitivity of these cultures. Objective To evaluate the GBS-Differential Agar (GBSDA) recently formulated by Becton Dickinson for the selective growth and production of orange colonies of b- hemolytic (b-H) GBS. Methods 283 vaginal swabs (VAG) collected from pregnant women were inoculated in selective Lim broth. After overnight incubation, Lim broth were subcultured on GBSDA, on Granada agar (Biomedics, Spain) and on Columbia blood agar (BA). To evaluate the stability, 99 isolates of GBS (REF) from adult or neonatal infections (Belgian GBS reference laboratory collection) were cultured on GBSDA and Granada at their limit of expiration, and on BA. GBSDA and Granada were incubated anaerobically and BA aerobically + 7% CO2, at 35°C, 24 to 48 h. Positive and negative control strains (GBS ; E. faecalis) were cultured with each run. Specific identification of colonies suggestive of GBS (pale to dark orange on GBSDA and Granada, b-H on BA) was performed. Results b-H GBS were recovered from 63 VAG (22.3 %): 62 were easily identified after overnight incubation on GBSDA and 63 on Granada without requiring any subculture. All GBS were also recovered from BA however it was after many subcultures. All orange colonies were confirmed as GBS. Among REF, 3 strains were non hemolytic ; they grew but were not differentiated as orange colonies on GBSDA or Granada. 96 REF were b-H, 94 (97.9%) produced orange to very dark orange colonies on GBSDA, 2 produced white colonies, and on Granada, 74 (77.1 %) produced pale to dark orange colonies and 22 white to white-orange colonies. Conclusion 1) GBSDA and Granada: a) very high sensitivity and specificity for the detection of b-H GBS, in a single step b) Results available within 48 h after inoculation in Lim broth, low workload 2) Excellent stability up to expiration date for GBSDA 3) Non hemolytic GBS: grown but not differentiated on GBSDA or Granada.
Research units :	Centre National de Référence des streptocoques du groupe B
Target :	Professionals
Permalink :	http://hdl.handle.net/2268/60849

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Open access	ASM 2004 poster GBSDA.pdf		Publisher postprint	384.89 kB	View/Open

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