ORBi: Servais Anne-Catherine - Influence of the BGE composition on analyte response in CD-mediated NACE-MS

Reference : Influence of the BGE composition on analyte response in CD-mediated NACE-MS


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/6082

Title :	Influence of the BGE composition on analyte response in CD-mediated NACE-MS
Language :	English
Author, co-author :	Servais, Anne-Catherine [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Mol, Roelof [Universiteit Utrecht > Biomedical Analysis > > >]
	Rousseau, Anne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Somsen, G. W. [Universiteit Utrecht > Biomedical Analysis > > >]
	de Jong, G. J. [Universiteit Utrecht > Biomedical Analysis > > >]
Publication date :	2010
Journal title :	Electrophoresis
Publisher :	Vch Publishers
Volume :	31
Issue/season :	7
Pages :	1157-1161
Audience :	International
ISSN :	0173-0835
City :	Weinheim
Country :	Germany
Keywords :	[en] Analyte ionization ; BGE composition ; CD ; MS ; NACE
Abstract :	[en] The influence of the BGE composition, including the addition of a single-isomer sulfated beta-CD derivative, on the ionization performance of the model compound carvedilol in NACE-ESI-MS was studied using an alternative infusion method. This approach employs voltage-induced infusion of the BGE containing the analyte, and takes into account the effects of variations in EOF and effective analyte mobility on the ESI-MS intensity. First, the optimal composition of the sheath liquid for CE-MS in terms of signal abundance and stability was determined. The BGE ammonium formate, acetate, and camphorsulfonate were found to have similar effects on analyte ionization. Addition of single-isomer sulfated beta-CD derivatives (available as sodium salt) to the BGE revealed that the anionic CD derivatives did not give rise to the same ionization suppression effect. This result can be attributed to differences in the dissociation state of these sodium salts. Finally, it is shown that information about chiral selectivity can also be obtained with the applied infusion method.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
Permalink :	http://hdl.handle.net/2268/6082

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