coccinellidae; alkaloid biosynthesis; labelling; glutamine; fat body
Abstract :
[en] In vitro experiments using [1-C-14] and [2-C-14] acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7-punctata, respectively. The labelled alkaloids obtained in these experiments had a specific activity about ten times higher than that of the samples obtained in feeding experiments. This in vitro assay has enabled us to demonstrate that these two alkaloids are most likely biosynthesised through a fatty acid rather than a polyketide pathway, that glutamine is the preferred source of the nitrogen atom and that alkaloid biosynthesis takes place in the insect fat body.
Disciplines :
Chemistry
Author, co-author :
Laurent, Pascal ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Braekman, Jean-Claude
Daloze, Désiré
Pasteels, Jacques
Language :
English
Title :
In vitro production of adaline and coccinelline, two defensive alkaloids from ladybird beetles (Coleoptera : Coccinellidae)
Publication date :
2002
Journal title :
Insect Biochemistry and Molecular Biology
ISSN :
0965-1748
eISSN :
1879-0240
Publisher :
Pergamon Press - An Imprint of Elsevier Science, Oxford, United Kingdom
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