Article (Scientific journals)
Cation Involvement in Telomestatin Binding to G-Quadruplex DNA
Rosu, Frédéric; Gabelica, Valérie; Smargiasso, Nicolas et al.
2010In Journal of Nucleic Acids, p. 121259
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Keywords :
G-quadruplex; mass spectrometry; electrospray; noncovalent; DNA; telomestatin; binding; cation
Abstract :
[en] The binding mode of telomestatin to G-quadruplex DNA has been investigated using electrospray mass spectrometry, by detecting the intact complexes formed in ammonium acetate. The mass measurements show the incorporation of one extra ammonium ion in the telomestatin complexes. Experiments on telomestatin alone also show that the telomestatin alone is able to coordinate cations in a similar way as a crown ether. Finally, density functional theory calculations suggest that in the G-quadruplex-telomestatin complex, potassium or ammonium cations are located between the telomestatin and a G-quartet. This study underlines that monovalent cation coordination capabilities should be integrated in the rational design of G-quadruplex binding ligands.
Research center :
Giga-Systems Biology and Chemical Biology - ULiège
Disciplines :
Chemistry
Biochemistry, biophysics & molecular biology
Author, co-author :
Rosu, Frédéric ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Gabelica, Valérie ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Smargiasso, Nicolas ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Center for Analytical Research and Technology (CART)
Shin-ya, Kazuo;  National Institute of Advanced Industrial Science and Technology, Japan
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Language :
English
Title :
Cation Involvement in Telomestatin Binding to G-Quadruplex DNA
Publication date :
2010
Journal title :
Journal of Nucleic Acids
ISSN :
2090-0201
eISSN :
2090-021X
Publisher :
SAGE-Hindawi Access to Research, London, United Kingdom
Special issue title :
G-quadruplexes
Pages :
121259
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 04 June 2010

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