Article (Scientific journals)
Performances of a multidimensional on-line SPE-LC-ECD method for the determination of three major catecholamines in native human urine: Validation, risk and uncertainty assessments
Rozet, Eric; Morello, Rosa; Lecomte, Frédéric et al.
2006In Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 844 (2), p. 251-260
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Keywords :
catecholamines; norepinephrine; epinephrine; dopamine; urine; on-line SPE; restricted access materials; RAM; multidimensional; ECD; columnswitching; validation; accuracy profile; risk analysis; measurement uncertainty
Abstract :
[en] A novel, multidimensional on-line SPE-LC method with electrochemical detection is described for the fully automated and direct analysis of the catecholamines norepinephrine, epinephrine and dopamine in urine. The integrated extractive clean-up of the raw biofluid is based on a SPE-column packed with restricted access material (RAM) which is modified with the affinity ligand nitrophenylboronic acid. The method was fully validated according to a recent approach based on an accuracy profile. The acceptance limits were set at +/- 15% of the nominal concentration values. The method was found accurate over a concentration range from 15 to 500 mu g/l for norepinephrine, from 5 to 500 mu g/l for epinephrine and from 50 to 500 mu g/l for dopamine. The relative risk for the use of the validated method in routine analysis was also assessed based on this validation strategy. It was found that at most 3.5% of future sample measurements will fall outside the acceptance limits. This demonstrates the high reliability of the analytical method described. Moreover, the measurements uncertainties were deduced from the validation experiments without any additional effort. (c) 2006 Elsevier B.V. All rights reserved.
Disciplines :
Chemistry
Biochemistry, biophysics & molecular biology
Author, co-author :
Rozet, Eric ;  Université de Liège - ULiège > Chimie analytique
Morello, Rosa;  University Hospital Grosshadern > Institute of Clinical Chemistry > Laboratory of BioSeparation
Lecomte, Frédéric  ;  Université de Liège - ULiège > Chimie analytique
Martin, Gaëlle B.;  Université de Liège - ULiège > Bioanalytical Chemistry Research Unit > Laboratory of Analytical Pharmaceutical Chemistry
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Boos, Karl-Siegfried;  University Hospital Grosshadern > Institute of Clinical Chemistry > Laboratory of BioSeparation
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Language :
English
Title :
Performances of a multidimensional on-line SPE-LC-ECD method for the determination of three major catecholamines in native human urine: Validation, risk and uncertainty assessments
Publication date :
05 December 2006
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
ISSN :
1570-0232
eISSN :
1873-376X
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
844
Issue :
2
Pages :
251-260
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 February 2009

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