Reference : Performances of a multidimensional on-line SPE-LC-ECD method for the determination of...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/6025
Performances of a multidimensional on-line SPE-LC-ECD method for the determination of three major catecholamines in native human urine: Validation, risk and uncertainty assessments
English
Rozet, Eric mailto [Université de Liège - ULg > > Chimie analytique >]
Morello, Rosa [University Hospital Grosshadern > Institute of Clinical Chemistry > Laboratory of BioSeparation >]
Lecomte, Frédéric mailto [Université de Liège - ULg > > Chimie analytique >]
Martin, Gaëlle B. [Université de Liège - ULg > Bioanalytical Chemistry Research Unit > Laboratory of Analytical Pharmaceutical Chemistry >]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Boos, Karl-Siegfried [University Hospital Grosshadern > Institute of Clinical Chemistry > Laboratory of BioSeparation >]
Hubert, Philippe mailto [Université de Liège - ULg > > Chimie analytique >]
5-Dec-2006
Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences
Elsevier Science Bv
844
2
251-260
Yes (verified by ORBi)
International
1570-0232
Amsterdam
[en] catecholamines ; norepinephrine ; epinephrine ; dopamine ; urine ; on-line SPE ; restricted access materials ; RAM ; multidimensional ; ECD ; columnswitching ; validation ; accuracy profile ; risk analysis ; measurement uncertainty
[en] A novel, multidimensional on-line SPE-LC method with electrochemical detection is described for the fully automated and direct analysis of the catecholamines norepinephrine, epinephrine and dopamine in urine. The integrated extractive clean-up of the raw biofluid is based on a SPE-column packed with restricted access material (RAM) which is modified with the affinity ligand nitrophenylboronic acid. The method was fully validated according to a recent approach based on an accuracy profile. The acceptance limits were set at +/- 15% of the nominal concentration values. The method was found accurate over a concentration range from 15 to 500 mu g/l for norepinephrine, from 5 to 500 mu g/l for epinephrine and from 50 to 500 mu g/l for dopamine. The relative risk for the use of the validated method in routine analysis was also assessed based on this validation strategy. It was found that at most 3.5% of future sample measurements will fall outside the acceptance limits. This demonstrates the high reliability of the analytical method described. Moreover, the measurements uncertainties were deduced from the validation experiments without any additional effort. (c) 2006 Elsevier B.V. All rights reserved.
Researchers ; Professionals
http://hdl.handle.net/2268/6025
10.1016/j.jchromb.2006.07.060

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