Reference : The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class a penicil...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/5994
The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class a penicillin-binding protein
English
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Hallut, Séverine [>ULg > > > > CIP > >]
Rhazi, Noureddine mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Amoroso, Ana Maria mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Bouillenne, Fabrice mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Coyette, Jacques mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Jul-2004
Journal of Bacteriology
Amer Soc Microbiology
186
13
4412-4416
Yes (verified by ORBi)
International
0021-9193
Washington
[en] Enterococcus faecalis ; PBP
[en] soluble derivative of the Enterococcus faecalis JH2-2 class A PBP1 (*PBP1) was overproduced and purified. It exhibited a glycosyltransferase activity on the Escherichia coli (14)C(-)labeled lipid 11 precursor. As a DD-peptidase, it could hydrolyze thiolester substrates with efficiencies similar to those of other class A penicillin-binding proteins (PBPs) and bind beta-lactams, but with k(2)/K (a parameter accounting for the acylation step efficiency) values characteristic of penicillin-resistant PBPs.
Researchers
http://hdl.handle.net/2268/5994
10.1128/JB.186.13.4412-4416.2004

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