Reference : The Penicillin Resistance of Enterococcus Faecalis Jh2-2r Results from an Overproduction...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/5993
The Penicillin Resistance of Enterococcus Faecalis Jh2-2r Results from an Overproduction of the Low-Affinity Penicillin-Binding Protein Pbp4 and Does Not Involve a Psr-Like Gene
English
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Sapunaric, Frédéric mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Amoroso, Ana Maria mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Thamm, Iris mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Coyette, Jacques mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Sep-2001
Microbiology
Society for General Microbiology
147
Pt 9
2561-9
Yes (verified by ORBi)
International
1350-0872
Reading RG7 1AG
UK
[en] Enterococcus faecalis ; résistance ; pénicilline
[en] A penicillin-resistant mutant, JH2-2r (MIC 75 microg ml(-1)), was isolated from Enterococcus faecalis JH2-2 (MIC 5 microg ml(-1)) by successive passages on plates containing increasing concentrations of benzylpenicillin. A comparison of the penicillin-binding protein (PBP) profiles in the two strains revealed a more intensely labelled PBP4 in JH2-2r. Because the sequences of the JH2-2 and JH2-2r pbp4 genes were strictly identical, even in their promoter regions, this intensive labelling could only be associated with an overproduction of the low-affinity PBP4. No psr gene analogous to that proposed to act as a regulator of PBP5 synthesis in Enterococcus hirae and Enterococcus faecium could be identified in the vicinity of pbp4 in E. faecalis JH2-2 and JH2-2r. However, a psr-like gene distant from pbp4 was identified. The cloning and sequencing of that psr-like gene from both E. faecalis strains indicated that they were identical. It is therefore postulated that the PBP4 overproduction in E. faecalis JH2-2r results from the modification of an as yet unidentified factor.
Researchers ; Professionals
http://hdl.handle.net/2268/5993

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