Reference : Murine bone marrow stromal cells sustain in vivo the survival of hematopoietic stem cell...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/59882
Murine bone marrow stromal cells sustain in vivo the survival of hematopoietic stem cells and the granulopoietic differentiation of more mature progenitors.
English
Hubin, Frederique mailto [Université de Liège - ULG > Des sciences biomédicales et précliniques > Cytologie et Histologie > >]
Humblet, Chantal mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Belaid, Zakia [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Lambert, Charles mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Boniver, Jacques mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Thiry, Albert mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Defresne, Marie-Paule mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
2005
Stem Cells
AlphaMed Company, Inc.
23
10
1626-33
Yes (verified by ORBi)
International
1066-5099
1549-4918
Miamisburg
OH
[en] in vivo model ; Adipocytes/cytology ; MS-5cell line ; stromal cells ; microenvironment ; hematopoiesis ; Animals ; Bone Marrow Cells/cytology ; Cell Differentiation ; Cell Line ; Cell Survival ; Cells, Cultured ; Fibroblasts/cytology/metabolism/transplantation ; Granulocytes/cytology ; Green Fluorescent Proteins/metabolism ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/cytology/metabolism ; Immunohistochemistry ; Mice ; Stromal Cells/cytology/transplantation ; Time Factors
[en] The study of the human hematopoietic system would be facilitated by availability of a relevant animal model. Because the medullar microenvironment is made of different types of cells, interactions between hematopoietic cells and stromal cells are difficult to analyze in detail. As an approach for establishing an in vivo model to dissect these interactions, we grafted murine bone marrow fibroblastic cells (MS-5 cell line) with hematopoietic cells into the kidney capsule of syngenic mice. To identify the origin of cells present in the graft, we used green fluorescent protein-stable transfected MS-5 cells for the transplantation. To analyze the evolution of stromal cells and identify hematopoietic cells able to develop in these conditions, we performed morphology, histochemistry, and immunohistology on tissue sections at different times after transplantation. When injected alone, MS-5 cells differentiate into adipocytes. When injected with a bone marrow suspension or with isolated CD45+ cells (leukocytes), the stromal cells keep their fibroblastic morphology and their alkaline phosphatase expression and sustain granulopoiesis. When injected with hematopoietic stem cells called c-kit+ Sca-1+ Lin- suspension, clusters of hematopoietic cells are also observed: They do not present any granulopoietic activity and do not belong to B or T population nor to erythroid lineage. They are quiescent, induce bone marrow recovery and survival of lethally irradiated recipients, are able to form macroscopic colonies in the spleen, and are able to form very few colonies in vitro, suggesting that they are hematopoietic stem cells. In conclusion, our results show that reticular fibroblastic stromal cells MS-5 sustain the survival of stem cells and are not able to induce their differentiation. However, they can control differentiation, proliferation, and/or survival of hematopoietic cells engaged in myeloid lineage.
Researchers
http://hdl.handle.net/2268/59882
also: http://hdl.handle.net/2268/74332
10.1634/stemcells.2005-0041

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