ORBi: Merino J. - Autoimmune syndrome after neonatal induction of tolerance to alloantigens: analysis of the role of donor T cells in the induction of autoimmunity

Reference : Autoimmune syndrome after neonatal induction of tolerance to alloantigens: analysis of t...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Genetics & genetic processes
	To cite this reference:	http://hdl.handle.net/2268/5925

Title :	Autoimmune syndrome after neonatal induction of tolerance to alloantigens: analysis of the role of donor T cells in the induction of autoimmunity
Language :	English
Author, co-author :	Merino, J. [Centre Medical Universitaire, Geneva, Switzerland > > > >]
	Schurmans, Stéphane [Université Libre de Bruxelles - ULB > > > > >]
	Wen, L. [Centre Medical Universitaire, Geneva, Switzerland > > > >]
	Brighouse, G. [Centre Medical Universitaire, Geneva, Switzerland > > > >]
	Luzuy, S. [Centre Medical Universitaire, Geneva, Switzerland > > > >]
	Lambert, P. H. [Centre Medical Universitaire, Geneva, Switzerland > > > >]
Publication date :	1990
Journal title :	Clinical & Experimental Immunology
Publisher :	Blackwell Publishing
Volume :	79
Pages :	273-278
Audience :	International
ISSN :	0009-9104
e-ISSN :	1365-2249
City :	Oxford
Country :	United Kingdom
Keywords :	[en] neonatal tolerance ; autoimmunity ; T cells
Abstract :	[en] The injection of (C57BL/6 x BALB/c) F1 spleen cells into BALB/c newborn mice leads to activation of persisting F1 donor B cells and development of a lupus-like syndrome in tolerized BALB/c mice. This syndrome is characterized by hypergammaglobulinaemia, high levels of anti-DNA and anti-Sm antibodies, circulating immune complexes and deposits of immunoglobulin in renal glomeruli. The role of donor T cells in this model was investigated by injecting the newborn mice with F1 cells depleted in different T cell subsets by using specific monoclonal antibodies (MoAbs). Tolerance, as shown by an absence of H-2b-specific CTL alloreactivity and persistence of immunoglobulin bearing the donor allotype were observed in mice injected with F1 cells previously depleted in the CD4+ and/or CD8+ T cell subsets as well as in those which received Thy-1+-depleted F1 spleen cells. In these mice, a typical autoimmune syndrome was found, including splenomegaly and lymphadenopathy, anti-ssDNA and anti-aortic myosin IgG antibodies and renal deposition of immunoglobulin. However, some quantitative changes were seen: the levels of anti-aortic myosin antibodies were lower in mice tolerized with CD4+-depleted F1 cells than in those receiving untreated F1 cells. Conversely, higher levels of these autoantibodies were observed in mice tolerized with CD8+-depleted F1 cells. These results suggest that mature donor T cells are not necessary neither for the establishment of neonatal tolerance to alloantigens nor for the activation of F1 donor B cells in the production of the autoimmune syndrome in tolerant mice, but they may contribute in the regulation of the expression of autoreactive B cell clones.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/5925

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