Reference : Mixed origin of neovascularization of human endometrial grafts in immunodeficient mou...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/5842
Mixed origin of neovascularization of human endometrial grafts in immunodeficient mouse models
English
Alvarez Gonzalez, Maria-Luz mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Frankenne, F. [> >]
Galant, C. [> >]
Marbaix, E. [> >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Nisolle, Michelle mailto [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
Beliard, Aude mailto [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
2009
Human Reproduction
Oxford University Press
24
9
2217-24
Yes (verified by ORBi)
International
0268-1161
1460-2350
Oxford
United Kingdom
[en] endometrium ; chimeric vessels ; fluorescence in-situ hybridization ; lectin ; angiogenesis
[en] BACKGROUND: In vivo mouse models have been developed to study the physiology of normal and pathologic endometrium. Although angiogenesis is known to play an important role in endometrial physiology and pathology, the origin of neovasculature in xenografts remains controversial. The aim of this study was to assess the origin of the neovasculature of endometrial grafts in different mouse models. METHODS: Human proliferative endometrium (n = 19 women) was grafted s.c. in two immunodeficient mouse strains: nude (n = 8) and severely compromised immunodeficient (SCID; n = 20). Mice were also treated with estradiol, progesterone or levonorgestrel. Fluorescence in-situ hybridization using a centromeric human chromosome X probe, immunohistochemistry (von Willebrand factor and collagen IV) and lectin perfusion were performed to identify the origin of the vessels. RESULTS: More than 90% of vessels within xenografts were of human origin 4 weeks after implantation. Some vessels (9.67 +/- 2.01%) were successively stained by human or mouse specific markers, suggesting the presence of chimeric vessels exhibiting a succession of human and murine portions. No difference in staining was observed between the two strains of mouse or different hormone treatments. Furthermore, erythrocytes were found inside human vessels, confirming their functionality. CONCLUSION: This article shows that human endometrial grafts retain their own vessels, which connect to the murine vasculature coming from the host tissue and become functional.
http://hdl.handle.net/2268/5842

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