Reference : International External Quality Assurance for Laboratory Identification and Typing of Str...
Scientific journals : Article
Life sciences : Microbiology
Human health sciences : Laboratory medicine & medical technology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/58298
International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (group B streptococci)
English
Afshar, Baharak [Health Protection Agency, Colindale, London, UK > > > >]
Broughton, Karen [Health Protection Agency, Colindale, London, UK > > > >]
Creti, Roberta [Istituto Superiore di Sanita, Rome, Italy > > > >]
Decheva, Antoaneta [National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria > > > >]
Hufnagel, Markus [University Medical Center Freiburg, Germany > Center for Pediatrics and Adolescent Medicine > > >]
Kriz, Paula [National Institute of Public Health, Prague, Czech Republic > > > >]
Lambertsen, Lotte [Statens Serum Institute, Copenhagen, Denmark > > > >]
Lovgren, Marguerite [Provincial Laboratory for Public Health, University of Alberta Hospital, Canada > > > >]
MELIN, Pierrette mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Orefici, Graziella [Istitute Superiore di Sanita, Rome, Italy > > > >]
Poyart, Claire [Hôpital Cochin, Université Paris Descartes, Paris, France > Centre national de référence des streptocoques > > >]
Radtke, Andreas [St Olavs University Hospital, Trondheim, Norway > > > >]
Rodriguez-Granger, Javier [Servicio Andaluz de Salud, Hospital Universitario Virgen de las Nieves, Granada, Sain > > > >]
Skov Sorensen, Uffe B [Aarhus Universitet - AU , Denmark > > > >]
Telford, John [Novartis Vaccines & Diagnostics, Siena, Italy > > > >]
Valinsky, Lea [National Streptococcus Reference Centre, Jerusalem, Israel > > > >]
Zachariadou, Levantia [Aghia Sophia Children's Hospital, Athens, greece > > > >]
Members of the DEVANI Study Group [> >]
Efstratiou, Androulla [Health Protection Agency, Colindale, London, UK > > > >]
16-Feb-2011
Journal of Clinical Microbiology
American Society for Microbiology (ASM)
Yes (verified by ORBi)
International
0095-1137
1098-660X
Washington
DC
[en] Streptococcus agalactiae ; Group B streptococci ; typing ; External Quality Assurance
[en] We report the results from the first international multicentre external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part of DEVANI (DEsign of a Vaccine Against Neonatal Infections), a pan-European programme. A questionnaire-based surveillance was undertaken amongst eight DEVANI and six non-DEVANI participating laboratories from 13 countries in order to assess their current microbiological procedures for GBS screening, diagnosis and typing. GBS strains from three EQA distributions were characterised using molecular and serological methods based on GBS capsular polysaccharide typing. Participants were asked to test the first distribution using their current serotyping and genotyping methods. Strep-B-Latex agglutination method was the most widely used method with a typeability value of >90%. A multiplex PCR assay for GBS capsular gene typing was also used by two of 14 centres who achieved a typeability value of 93%; this assay only detected 9 of 10 GBS capsular polysaccharide genes. From the second and third EQA studies standardised protocols were prepared for serological and molecular typing of GBS strains based on the Strep-B-Latex agglutination method and a novel multiplex PCR assay which detected all ten GBS capsular types (Ia to IX). These standardised protocols are being used by many European laboratories and as the use of these methods increase, it is imperative to continuously improve and assess laboratory performance and offer training to any laboratories that have technical difficulties.
Centre national de référence des streptocoques du groupe B
Union Européenne = European Union - UE = EU
DEVANI: Design of a Vaccine Against Neonatal Infections
Researchers ; Professionals ; Others
http://hdl.handle.net/2268/58298

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