ORBi: Afshar Baharak - International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (group B streptococci)

Reference : International External Quality Assurance for Laboratory Identification and Typing of Str...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Human health sciences : Laboratory medicine & medical technology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/58298

Title :	International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (group B streptococci)
Language :	English
Author, co-author :	Afshar, Baharak [Health Protection Agency, Colindale, London, UK > > > >]
	Broughton, Karen [Health Protection Agency, Colindale, London, UK > > > >]
	Creti, Roberta [Istituto Superiore di Sanita, Rome, Italy > > > >]
	Decheva, Antoaneta [National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria > > > >]
	Hufnagel, Markus [University Medical Center Freiburg, Germany > Center for Pediatrics and Adolescent Medicine > > >]
	Kriz, Paula [National Institute of Public Health, Prague, Czech Republic > > > >]
	Lambertsen, Lotte [Statens Serum Institute, Copenhagen, Denmark > > > >]
	Lovgren, Marguerite [Provincial Laboratory for Public Health, University of Alberta Hospital, Canada > > > >]
	MELIN, Pierrette [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
	Orefici, Graziella [Istitute Superiore di Sanita, Rome, Italy > > > >]
	Poyart, Claire [Hôpital Cochin, Université Paris Descartes, Paris, France > Centre national de référence des streptocoques > > >]
	Radtke, Andreas [St Olavs University Hospital, Trondheim, Norway > > > >]
	Rodriguez-Granger, Javier [Servicio Andaluz de Salud, Hospital Universitario Virgen de las Nieves, Granada, Sain > > > >]
	Skov Sorensen, Uffe B [Aarhus Universitet - AU , Denmark > > > >]
	Telford, John [Novartis Vaccines & Diagnostics, Siena, Italy > > > >]
	Valinsky, Lea [National Streptococcus Reference Centre, Jerusalem, Israel > > > >]
	Zachariadou, Levantia [Aghia Sophia Children's Hospital, Athens, greece > > > >]
	Members of the DEVANI Study Group [> >]
	Efstratiou, Androulla [Health Protection Agency, Colindale, London, UK > > > >]
Publication date :	16-Feb-2011
Journal title :	Journal of Clinical Microbiology
Publisher :	American Society for Microbiology (ASM)
Audience :	International
ISSN :	0095-1137
e-ISSN :	1098-660X
City :	Washington
Country :	DC
Keywords :	[en] Streptococcus agalactiae ; Group B streptococci ; typing ; External Quality Assurance
Abstract :	[en] We report the results from the first international multicentre external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part of DEVANI (DEsign of a Vaccine Against Neonatal Infections), a pan-European programme. A questionnaire-based surveillance was undertaken amongst eight DEVANI and six non-DEVANI participating laboratories from 13 countries in order to assess their current microbiological procedures for GBS screening, diagnosis and typing. GBS strains from three EQA distributions were characterised using molecular and serological methods based on GBS capsular polysaccharide typing. Participants were asked to test the first distribution using their current serotyping and genotyping methods. Strep-B-Latex agglutination method was the most widely used method with a typeability value of >90%. A multiplex PCR assay for GBS capsular gene typing was also used by two of 14 centres who achieved a typeability value of 93%; this assay only detected 9 of 10 GBS capsular polysaccharide genes. From the second and third EQA studies standardised protocols were prepared for serological and molecular typing of GBS strains based on the Strep-B-Latex agglutination method and a novel multiplex PCR assay which detected all ten GBS capsular types (Ia to IX). These standardised protocols are being used by many European laboratories and as the use of these methods increase, it is imperative to continuously improve and assess laboratory performance and offer training to any laboratories that have technical difficulties.
Research units :	Centre national de référence des streptocoques du groupe B
Funders :	Union Européenne = European Union - UE = EU
Name of the research project :	DEVANI: Design of a Vaccine Against Neonatal Infections
Target :	Researchers ; Professionals ; Others
Permalink :	http://hdl.handle.net/2268/58298

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