[en] Metabolism of muscle growth in the hindquarter was investigated by the arterio-venous difference (AVD) technique in Belgian Blue double-muscled type bulls at maintenance or at fattening. The bulls were fitted with an aortic ultrasonic blood flow probe and with catheters in the aorta and vena cava. They were offered a diet allowing for maintenance (MP) during a period of 15 d, at the end of which measurements were made over 3 d. Bulls were then given a fattening diet (FP) and the measurements were repeated. Arterial blood flow was approximately 1 L/min greater when the bulls were standing than when lying. Blood flow was 2 L/min higher during FP than during MP. The AVD and uptake of glucose were maximal at 1400 and 1600. Uptake of alpha-amino nitrogen decreased immediately after a meal. The increase in glucose from MP to FP fitted very well with the calculated energy needs for muscle growth. The AVD and uptake of alpha-amino nitrogen, total amino acids, and total nonessential amino acids were negative during MP and positive and significantly higher during FP. There was also a significant increase in AVD and uptake of essential and branched-chain amino acids when the bulls were changed from MP to FP. When changing from maintenance to fattening, the incremental glucose and amino acid hindquarter uptake provided energy and supply for muscle protein accretion, respectively. The level of alanine transamination was also sharply reduced.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Hornick, Jean-Luc ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Van Eenaeme, Christian ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Diez, Marianne ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Minet, V.
Istasse, Louis ; Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Language :
English
Title :
Comparison of hindquarter metabolite uptakes in Belgian Blue double-muscled bulls at maintenance or during fattening.
Publication date :
1997
Journal title :
Journal of Animal Science
ISSN :
0021-8812
eISSN :
1525-3163
Publisher :
American Society of Animal Science, Savoy, United States - Illinois
Bell, A. W., T. E. Hilditch, and G. E. Thompson. 1976. The distribution of blood flow between individual muscles and non-muscular tissues in the hindlimb of the young ox (Bos taurus): values at thermoneutrality and during exposure to cold. J. Physiol. 257:229-243.
Bergman, E. N., and R. N. Heitmann. 1978. Metabolism of amino acids by the gut, liver, kidneys, and peripheral tissues. Fed. Proc. 37:1228-1232.
Boisclair, Y. R., A. W. Bell, F. R. Dunshea, M. Harkins, and D. E. Bauman. 1993. Evaluation of the arteriovenous difference technique to simultaneously estimate protein synthesis and degradation in the hindlimb of fed and chronically underfed steers. J. Nutr. 123:1076-1088.
Buttery, P. J., and E. F. Annison. 1973. The Biological Efficiency of Protein Production. p 148. Cambridge University Press, Cambridge, U.K.
Chang, T.W., and A. L. Goldberg. 1978. The origin of alanine produced in skeletal muscle. J. Biol. Chem. 253:3677-3684.
Clark, V. L., and J. A. Kruse. 1992. Arterial catheterisation. Crit. Care Clin. 8:687-697.
Coward, B. J., and P. J. Buttery. 1982. Metabolism of perfused ruminant muscle. J. Agric. Sci. 98:307-316.
Coward, J., and P. J. Buttery. 1979. Catabolism of branched chain amino acids by ruminant muscle. Proc. Nutr. Soc. 38:139A.
Degrès, J., D. Boisson, and P. Padieu. 1979. Gas-liquid chromatography of isobutyl ester, N(O)-heptafluorobutyrate derivatives of amino acids on a glass capillary column for quantitative separation in clinical biology. J. Chromatogr. Biomed. Appl. 162: 133-152.
Early, R. J., J. R. Thompson, R. J. Christopherson, and G. W. Sedwick. 1987. Blood branched-chain amino and aketo acid concentrations and net exchange across the portal-drained viscera and hindlimb of fed and fasted ruminants. Can. J. Anim. Sci. 67:1011-1020.
Eisemann, J. H., G. B. Huntington, and D. R. Catherman. 1996. Patterns of nutrient interchange and oxygen use among portal-drained viscera, liver, and hindquarters of beef steers from 235 to 525 kg body weight. J. Anim. Sci. 74:1812-1831.
Eisemann, J. H., G. B. Huntington, and C. L. Ferrell. 1988. Effects of dietary clenbuterol on metabolism of the hindquarters in steers. J. Anim. Sci. 66:342-353.
Felig, P. 1975. Amino acid metabolism in man. Annu. Rev. Biochem. 44:933-955.
Goldberg, A. L., and T. W. Chang. 1978. Regulation and significance of amino acid metabolism in skeletal muscle. Fed. Proc. 37: 2301-2307.
Harris, P. M., J. Lee, B. R. Sinclair, B. P. Treloar, and M. P. Gurnsey. 1994. Effect of food intake on energy and protein metabolism in the skin of Romney sheep. Br. J. Nutr. 71: 647-660.
Harris, P. M., P. A. Skene, V. Buchan, E. Milne, A. G. Calder, S. E. Anderson, A. Connell, and G. E. Lobley. 1992. Effect of food intake on hind-limb and whole-body protein metabolism in young growing sheep-Chronic studies based on arterio-venous techniques. Br. J. Nutr. 68:389-407.
Heitmann, R. N., and E. N. Bergman. 1980a. Integration of amino acid metabolism in sheep: effects of fasting and acidosis. Am. J. Physiol. 239:E248-E254.
Heitmann, R. N., and E. N. Bergman. 1980b. Transport of amino acids in whole blood and plasma of sheep. Am. J. Physiol. 239: E242-E247.
Henry, R. J., D. C. Cannon, and J. W. Winkelman. 1974. Clinical Chemistry. Principles and Technics. pp 1289-1292. Harper & Row, New York.
Hocquette, J.F., and M. Balage. 1996. Facilitative glucose transporters in ruminant. Proc. Nutr. Soc. 55:221-236.
Hornick, J. L., C. Van Eenaeme, S. Gauthier, P. Baldwin, and L. Istasse. 1996. Glucose, alpha-amino nitrogen, and amino acid exchange across the hindlimb in young double-muscled type bulls maintained at two growth rates. Can. J. Anim. Sci. 76: 193-202.
Istasse, L., F. D. D. Hovell, N. A. Macleod, and E. R. Orskov. 1987. The effects of continuous or intermittent infusion of propionic acid on plasma insulin and milk yield in dairy cows nourished by intragastric infusion of nutrients. Livest. Prod. Sci. 16: 201-214.
Lindsay, D. B., and P. J. Buttery. 1980. Metabolism in muscle. In: P. J. Buttery and D. B. Lindsay (Ed.) Protein Deposition in Animals. p 125. Butterworth, London.
Mayombo, A. P., I. Dufrasne, J. L. Hornick, M. Diez, and L. Istasse. 1997. Influence du stade de maturité de la plante de maïs récoltée pour ensilage sur la composition, le digestibilité apparente, les caractéristiques de fermentation dans le rumen et les performances zootechniques chez le taurillon à l'engrais. Ann. Zootech. 46:43-55.
McCormick, M. E., and K. E. Webb. 1982. Plasma free, erythrocyte free and plasma peptide amino acid exchange of calves during steady state and fasting metabolism. J. Nutr. 112:276-282.
Millward, D. J., and P. J. Garlick. 1976. The energy cost of growth. Proc. Nutr. Soc. 35:339-349.
Minet, V., C. Van Eenaeme, P. Raskin, I. Dufrasne, A. Clinquart, J. L. Hornick, M. Diez, A. P. Mayombo, P. Baldwin, J. M. Bienfait, and L. Istasse. 1996. Stratégies d'engraissement du taurillon Blanc Bleu belge culard. Performances, qualité des carcasses et de la viande, approche métabolique et bilan économique. Ministère des Classes Moyennes et de l'Agriculture, Brussels.
Odessey, R., and A. L. Goldberg. 1979. Leucine degradation in cell-free extracts of skeletal muscle. Biochem. J. 178:475-489.
Ortigues, I., C. Martin, and D. Durand. 1996. Circadian changes in net nutrient fluxes across the portal-drained viscera, the liver, and the hindquarters in preruminant calves. J. Anim. Sci. 74: 895-907.
Palmer, D. W., and J. T. Peters. 1969. Automated determination of free amino groups in serum and plasma using 2,4,6-trinitrobenzene sulfonate. Clin. Chem. 19:891-901.
Pethick, D. W., and B. Vernau. 1984.The effect of acetate on the utilisation of glucose by skeletal muscle of the sheep hindlimb. Can. J. Anim. Sci. 64(Suppl):291-292.
Picard, B., J. F. Hocquette, F. Bornes, S. Brazi, M. Vermorel, and Y. Geay. 1994. Muscle metabolism in normal and double-muscled calves: fiber characteristics and glucose transport rate. In: Proc. 13th Symp. Energy Metab. Farm Animals, September 18-24, Mojacar, Spain. pp 97-100.
Van Eenaeme, C., J. L. Hornick, P. Baldwin, N. Korsak, and L. Istasse. 1995. Assessment of amino acid nutritional status in young Belgian Blue bulls at different growth rates. Amino Acids 9:43.
Van Eenaeme, C., L. Istasse, P. Baldwin, A. Gabriel, V. de Haan, and J. M. Bienfait. 1989. Muscle protein turnover in young bulls in relation to breed and hormonal status, Asian-Australas. J. Anim. Sci. 2:200-201.
Webster, A.J.F., G. E. Lobley, P. J. Reeds, and J. D. Pullar. 1978. Protein mass, protein synthesis and heat loss in the Zucker rat. Proc. Nutr. Soc. 37:21A.
Weijs, P.J.M., V.V.A.M. Schreurs, R. E. Koopmanschap, H. N. A. Grooten, A. T. Schoonman, and H. A. Boekholt. 1993. Effects of acute and chronic level of protein supply on metabolic leucine utilisation in growing and mature rats. Br. J. Nutr. 70:117-125.
Wright, I.A., and A.J.F. Russel. 1991. Changes in the body composition of beef cattle during compensatory growth. Anim. Prod. 52: 105-113.