The IGF system in in-vitro human decidualization

[en] Decidualization of endometrial stromal cells (ESCs) is critical for a successful pregnancy but the molecular mechanisms of the process are poorly understood. In this study, we investigated whether the insulin-like growth factor (IGF) network is involved in this cellular process. Expression kinetics of members of the IGF system was examined at both mRNA and protein levels during in-vitro decidualization of cultured human ESCs. We found a significant up-regulation of IGF-II as well as of IGF-I receptor and the A and B insulin receptor (InsR) isoforms. In addition, levels of the key adaptor proteins insulin receptor substrate 1 (IRS-1) and IRS-2 increased, suggesting a potential involvement of the IGF signalling pathway in the decidualization process. Expression of two IGF binding proteins, IGFBP-1 and IGFBP-4, which can inhibit IGF action, also increased. In order to determine whether IGF signalling was activated during decidualization, the phosphorylation status of the receptors and the adaptor proteins was estimated. Only IRS-2 was slightly phosphorylated in decidualized cells and was further activated by the addition of exogenous IGF-II. These results suggest that the IGF signalling pathway could play a crucial role in the functions of decidualized endometrial cells

Research center :

Giga-Cancer - ULiège

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Ganeff, Corinne ; Université de Liège - ULiège > GIGA-R : Virologie - Immunologie

Chatel, G.

Munaut, Carine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Frankenne, F.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement

Winkler, Rose ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Language :

English

Title :

The IGF system in in-vitro human decidualization

Publication date :

2009

Journal title :

Molecular Human Reproduction

ISSN :

1360-9947

eISSN :

1460-2407

Publisher :

Oxford University Press, Oxford, United Kingdom

Volume :

Issue :

Pages :

27-38

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 January 2010

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184 (2 by ULiège)

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