ORBi: Saegerman Claude - Bovine brucellosis diagnosis by skin test: conditions for its use and evaluation of its performance

Reference : Bovine brucellosis diagnosis by skin test: conditions for its use and evaluation of its ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/57794

Title :	Bovine brucellosis diagnosis by skin test: conditions for its use and evaluation of its performance
Language :	English
Author, co-author :	Saegerman, Claude [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér. >]
	Vo, T. K. O. [ > > ]
	De Waele, L. [ > > ]
	Gilson, D. [ > > ]
	Bastin, A. [ > > ]
	Dubray, G. [ > > ]
	Flanagan, P. [ > > ]
	Limet, J. N. [ > > ]
	Letesson, J. J. [ > > ]
	Godfroid, J. [ > > ]
Publication date :	1999
Journal title :	Veterinary Record : Journal of the British Veterinary Association
Publisher :	British Veterinary Medicine
Volume :	145
Pages :	214-218
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0042-4900
City :	London
Country :	United Kingdom
Abstract :	[en] Brucellergene OCB (Rhône-Mérieux) was used as an allergen to define the intrinsic parameters of a skin test and to compare its properties with serology for the diagnosis of bovine brucellosis. The skin test was also evaluated for its capacity to solve problems associated with false positive reactions in serological tests. The optimal reading delay for the skin test was 72 hours. The brucellosis allergic reaction was two to three times less intense than the tuberculosis allergic reaction. An increase of 1.1 mm or more in the skin thickness was therefore considered to be an adequate cut-off. The specificity calculated for 1192 brucellosis-free animals (including animals from brucellosis-free herds in which false positive serological reactions had been reported) was 99-83 per cent (95 per cent confidence interval [CI] 99-40 to 99-98 per cent). The sensitivity determined from 27 experimentally infected heifers ranged from 93 per cent (95 per cent CI 76 to 100 per cent) to 78 per cent (95 per cent CI 58 to 91 per cent) when measured respectively one and six months after the infection. Allergic reactions could be detected in vaccinated animals up to four-and-a-half years after the vaccination. On the other hand, no sensitisation was recorded in naïve animals after up to eight monthly injections of the allergen. The skin test gave valuable information, in combination with the serological tests, in both acute and chronic brucellosis. The skin test discriminated brucellosis clearly from false positive serological reactions due to infections with Yersinia enterocolitica O9.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/57794

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