Reference : Interleukin-6 receptor shedding is enhanced by interleukin-1beta and tumor necrosis fact...
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/56818
Interleukin-6 receptor shedding is enhanced by interleukin-1beta and tumor necrosis factor alpha and is partially mediated by tumor necrosis factor alpha-converting enzyme in osteoblast-like cells.
English
Franchimont, Nathalie [> > > >]
Lambert, Cécile mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Huynen, Pascale mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Ribbens, Clio mailto [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
Relic, Biserka [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
Chariot, Alain [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Bours, Vincent mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
Merville, Marie-Paule mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Malaise, Michel mailto [Centre Hospitalier Universitaire de Liège - CHU > > Rhumatologie >]
2005
Arthritis and Rheumatism
Wiley Liss, Inc.
52
1
84-93
Yes (verified by ORBi)
International
0004-3591
1529-0131
New York
NY
[en] ADAM Proteins ; Cell Line, Tumor ; Humans ; Interleukin-1/pharmacology ; Metalloendopeptidases/genetics/metabolism ; Osteoblasts/metabolism ; RNA, Messenger/metabolism ; RNA, Small Interfering/pharmacology ; Receptors, Interleukin-6/biosynthesis/chemistry/genetics/metabolism ; Recombinant Proteins/pharmacology ; Solubility ; Transfection ; Tumor Necrosis Factor-alpha/pharmacology
[en] OBJECTIVE: Interleukin-6 (IL-6) and soluble IL-6 receptor (sIL-6R) activation of gp130 represents an alternative pathway for osteoclast development in inflammatory conditions. The goal of the present study was to investigate changes in sIL-6R levels in response to the inflammatory cytokines IL-1beta and tumor necrosis factor alpha (TNFalpha) and to determine the role of TNFalpha-converting enzyme (TACE) in this process. METHODS: Levels of sIL-6R in the culture media of MG63 and SAOS-2 osteoblast-like cell lines after exposure to various agents were determined by immunoassay. TACE protein levels were measured by Western immunoblotting. Cells were transfected with small interfering RNA (siRNA) or with an expression plasmid for IL-6R and TACE to determine the potential involvement of TACE in IL-6R shedding. RESULTS: IL-1beta and TNFalpha increased the levels of sIL-6R in the culture media of MG63 osteoblast-like cells. This effect was not influenced by cycloheximide or 5,6-dichlorobenzimidazole riboside but was markedly inhibited by the calcium chelator EGTA and by the TACE and matrix metalloproteinase inhibitor hydroxamate (Ru36156). IL-1beta and TNFalpha had no influence on the alternatively spliced form of IL-6R RNA. Levels of sIL-6R were reduced when MG63 cells were transiently transfected with TACE siRNA. Transfection of SAOS-2 cells with expression plasmids for IL-6R and TACE produced a dose-dependent increase in sIL-6R levels. CONCLUSION: IL-1beta- and TNFalpha-mediated induction of IL-6R shedding in osteoblast-like cells is at least partly dependent on TACE activation.
Giga-Signal Transduction
FNRS, TELEVIE, ARC ULG
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/56818
also: http://hdl.handle.net/2268/3731
10.1002/art.20727

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