Article (Scientific journals)
Glutathione-S-transferase pi expression in toxic epidermal necrolysis: a marker of putative oxidative stress in keratinocytes.
Paquet, Philippe; Pierard, Gérald
2007In Skin Pharmacology and Physiology, 20 (2), p. 66-70
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Keywords :
Adult; Aged; Biological Markers/metabolism; Epidermal Necrolysis, Toxic/enzymology; Female; Glutathione S-Transferase pi/biosynthesis; Humans; Immunohistochemistry; Keratinocytes/enzymology; Male; Middle Aged; Oxidative Stress; Pemphigoid, Bullous/enzymology
Abstract :
[en] BACKGROUND: Toxic epidermal necrolysis (TEN) is a dramatic drug-induced emergency related to extensive destruction of the epidermis. There is evidence that its pathomechanism involves impaired detoxication of xenobiotics. Glutathione-S-transferase pi (GST-pi) is a phase II detoxifying enzyme involved in drug metabolization by human keratinocytes. METHOD: Immunohistochemistry was performed in order to assess the expression of GST-pi in keratinocytes of TEN, other cutaneous adverse drug reactions and bullous pemphigoid. RESULTS: GST-pi was disclosed in the involved epidermis of 16/16 TEN patients. It was present in the cytoplasm of suprabasal keratinocytes. GST-pi was also expressed in the clinically uninvolved skin in a majority (8/12) of TEN patients. By contrast, it was rarely and poorly expressed in the other tested dermatoses. CONCLUSION: The pathomechanism of TEN is not related to an impaired quantitative expression of GST-pi. GST-pi expression is an early event in TEN. As oxidative stress is a major inducer of GST-pi, this mechanism might be involved in TEN. Its GST-pi expression mainly restricted to the suprabasal keratinocytes suggests that the pathomechanisms leading to keratinocyte death in TEN are distinct at different levels of the epidermis.
Disciplines :
Dermatology
Author, co-author :
Paquet, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Pierard, Gérald ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Language :
English
Title :
Glutathione-S-transferase pi expression in toxic epidermal necrolysis: a marker of putative oxidative stress in keratinocytes.
Publication date :
2007
Journal title :
Skin Pharmacology and Physiology
ISSN :
1660-5527
eISSN :
1660-5535
Publisher :
S. Karger, Basel, Switzerland
Volume :
20
Issue :
2
Pages :
66-70
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 February 2009

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