Metabolomic analysis of Strychnos nux-vomica, Strychnos icaja and Strychnos ignatii extracts by H-1 nuclear magnetic resonance spectrometry and multivariate analysis techniques
Frederich, Michel; Choi, Young Hae; Angenot, Lucet al.
[en] H-1 Nuclear magnetic resonance spectrometry and multivariate analysis techniques were applied for the metabolic profiling of three Strychnos species: Strychnos nux-vomica (seeds, stem bark, root bark), Strychnos ignatii (seeds), and Strychnos icaja (leaves, stem bark, root bark, collar bark). The principal component analysis (PCA) of the H-1 NMR spectra showed a clear discrimination between all samples, using the three first components. The key compounds responsible for the discrimination were brucine, loganin, fatty acids.. and Strychnos icaja alkaloids such as icajine and sungucine. The method was then applied to the classification of several "false angostura" samples. These samples were, as expected, identified as S. nux-vomica by PCA, but could not be clearly discriminated as root bark or stem bark samples after further statistical analysis. (C) 2004 Published by Elsevier Ltd.
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Choi, Young Hae
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Harnischfeger, Goetz
Lefeber, Alfons W. M.
Verpoorte, Robert
Language :
English
Title :
Metabolomic analysis of Strychnos nux-vomica, Strychnos icaja and Strychnos ignatii extracts by H-1 nuclear magnetic resonance spectrometry and multivariate analysis techniques
Publication date :
July 2004
Journal title :
Phytochemistry
ISSN :
0031-9422
eISSN :
1873-3700
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
Ambasta, S.S.P., 1986. The Useful Plants of India. Publications and Information Directorate, New Delhi, pp. 604-606
Bailey N.J.C., Sampson J., Hylands P.J., Nicholson J.K., Holmes E. Multi-component metabolic classification of commercial feverfew preparations via high-field 1H NMR spectroscopy and chemometrics. Planta Medica. 68:2002;734-738
Baser K.H.C., Bisset N.G. Alkaloids of Sri Lankan Strychnos nux-vomica. Phytochemistry. 21:1982;1423-1429
Bisset N.G. The Asian species of Strychnos. Part III. The ethnobotany. Lloydia. 37:1974;62-107
Bisset N.G. The Asian species of Strychnos. Part IV. The alkaloids. Lloydia. 39:1976;263-315
Bisset N.G., Choudhury A.K. Alkaloids from the leaves of Strychnos wallichiana. Phytochemistry. 13:1974;259-263
Bisset N.G., Walker M.D. Alkaloids from the stem bark of Strychnos ignatii. Phytochemistry. 13:1974;525-526
Bratati D., Bisset N.G. Alkaloids of Strychnos ignatii. Planta Medica. 56:1990;133
Bundy J.G., Spurgeon D.J., Svendsen C., Hankard P.K., Osborn D., Lindon J.C., Nicholson J.K. Earthworm species of the genus Eisenia can be phenotypically differentiated by metabolic profiling. FEBS Letters. 521:2002;115-120
Denoel A. Le Strychnos icaja Baillon du Congo Belge. Journal de Pharmacie de Belgique. 5:1950;59-77
Dymock, W., Warden, C.J.H., Hooper, D., 1891. Pharmacographia Indica. A History of the Principal Drugs of Vegetable Origin. Trench, Trübner & Co, London, pp. 458-505
Eriksson L., Johansson E., Kettaneh-Wold N., Wold S. Multi- and Megavariate Data Analysis. 2001;Umetrics Academy, Umeå, Sweden
Felter H.W., Lloyd J.U. King's American Dispensatory. 1898;Ohio Valley Co., Cincinnati
Flückiger F.A., Hanbury D. Histoire des Drogues d'Origine Végétale. 1878;Octave Doin, Paris
Frédérich M., De Pauw M., Prosperi C., Tits M., Brandt V., Penelle J., Hayette M., DeMol P., Angenot L. Strychnogucines A and B, two new antiplasmodial bisindole alkaloids from Strychnos icaja. Journal of Natural Products. 64:2001;12-16
Frédérich M., Depauw-Gillet M.C., Llabres G., Tits M., Hayette M.P., Brandt V., Penelle J., De Mol P., Angenot L. New anti-malarial and cytotoxic sungucine derivatives from Strychnos icaja roots. Planta Medica. 66:2000;262-269
Gadi-Biala R., Tits M., Wauters J.N., Angenot L. A new HPLC method for the assay of alkaloids in Strychnos nux-vomica and Strychnos ignatii. Fitoterapia. 67:1996;163-165
Gathercoal E.N., Wirth E.H. Pharmacognosy. 1947;Lea and Fegiger, Philadelphia
Grieve, M., 1971. A Modern Herbal, vol. 1, Dover Publications, New York, pp. 51-53
Iwu, M., 1993. Handbook of African Medicinal Plants. CRC Press, Boca Raton, pp. 244-245
Jacquemond-Collet I., Hannedouche S., Fabre N., Fouraste I., Moulis C. Two tetrahydroquinoline alkaloids from Galipea officinalis. Phytochemistry. 51:1999;1167-1169
Kambu K., Coune C., Angenot L. Nouveaux alcaloïdes des racines du Strychnos icaja. Planta Medica. 37:1980;161-164
Krukoff B.A., Marini-Bettolo G.B., Bisset N.G. American species of Strychnos. Lloydia. 35:1972;193-271
Marini-Bettolo G.B., Ciasca M.A., Galeffi C., Bisset N.G., Krukoff B.A. The occurrence of strychnine and brucine in an American species of Strychnos. Phytochemistry. 11:1972;381-384
Neuwinger H.D. African Ethnobotany: Poisons and Drugs, Chemistry, Pharmacology, Toxicology. 1996;Chapman & Hall, London
Perrot E. Matières Premières Usuelles du Règne Végétal. 1943;Masson, Paris
Philippe G., De Mol P., Zeches-Hanrot M., Nuzillard J.M., Tits M.H., Angenot L., Frederich M. Indolomonoterpenic alkaloids from Strychnos icaja roots. Phytochemistry. 62:2003;623-629
Schaneberg B.T., Crockett S., Bedir E., Khan I.A. The role of chemical fingerprinting: application to Ephedra. Phytochemistry. 62:2003;911-918
Sefcovic P., Dubravkova L., Torto F.G. Die Alkaloide der Blätter von Strychnos nux-vomica L. Planta Medica. 16:1968;143-146
Shaw F.H., de la Lande J.S. The alkaloids of Strychnos lucida. Australian Journal of Experimental Biological and Medicinal Sciences. 26:1948;199-203
Sumner L.W., Mendes P., Dixon R.A. Plant metabolomics: large-scale phytochemistry in the functional genomics era. Phytochemistry. 62:2003;817-836
Tschirch A. Handbuch der Pharmakognosie. 1923;Verlag von Chr. Herm. Tauchnitz, Leipzig
Ward J., Harris C., Lewis J., Beale H. Assessment of 1H NMR spectroscopy and multivariate analysis as a technique for metabolite fingerprinting of Arabidopsis thaliana. Phytochemistry. 62:2003;949-957
