Reference : PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Materials science & engineering
http://hdl.handle.net/2268/5542
PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination
English
Garinot, Marie [The Catholic University of Louvain (UCL) > > Unité de Pharmacie Galénique > >]
Fievez, Virginie [The Catholic University of Louvain (UCL) > > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire > >]
Pourcelle, Vincent [The Catholic University of Louvain (UCL) > > Unité de Chimie Organique et Médicinale > >]
Stoffelbach, François [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
des Rieux, Anne [The Catholic University of Louvain (UCL) > > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire > >]
Plapied, Laurence [The Catholic University of Louvain (UCL) > > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire > >]
Theate, Ivan [The Catholic University of Louvain (UCL), Cliniques Universitaires Saint-Luc > Department of Pathology > > >]
Freichels, Hélène [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Jérôme, Christine mailto [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Marchand-Brynaert, Jacqueline [The Catholic University of Louvain (UCL) > > Unité de Chimie Organique et Médicinale > >]
Schneider, Yves-Jacques [The Catholic University of Louvain (UCL) > > Unité de Chimie Organique et Médicinale > >]
Preat, Véronique [The Catholic University of Louvain (UCL) > > Unité de Pharmacie Galénique > >]
31-Jul-2007
Journal of Controlled Release
Elsevier Science Bv
120
3
195-204
Yes (verified by ORBi)
International
0168-3659
Amsterdam
[en] biomaterial ; nanomedicine ; carbon nanoparticle ; self-assembly in solution
[en] To improve the efficiency of orally delivered vaccines, PEGylated PLGA-based nanoparticles displaying RGD molecules at their surface were designed to target human M cells. RGD grafting was performed by an original method called "photografting" which covalently linked RGD peptides mainly on the PEG moiety of the PCL-PEG, included in the formulation. First, three non-targeted formulations with size and zeta potential adapted to M cell uptake and stable in gastro-intestinal fluids, were developed. Their transport by an in vitro model of the human Follicle associated epithelium (co-cultures) was largely increased as compared to mono-cultures (Caco-2 cells). RGD-labelling of nanoparticles significantly increased their transport by co-cultures. due to interactions between the RGD ligand and the I intregrins detected at the apical surface of co-cultures. In vivo studies demonstrated that RGD-labelled nanoparticles particularly concentrated in M cells. Finally, ovalbumin-loaded nanoparticles were orally administrated to mice and induced an IgG response, attesting antigen ability to elicit an immune response after oral delivery.
Center for Education and Research on Macromolecules (CERM)
Researchers
http://hdl.handle.net/2268/5542
10.1016/j.jconrel.2007.04.021
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3D-4NSWYVS-1-F&_cdi=4944&_user=532038&_orig=browse&_coverDate=07%2F31%2F2007&_sk=998799996&view=c&wchp=dGLbVtz-zSkWz&md5=c80f62e8d6188494cf754904868425f0&ie=/sdarticle.pdf
http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/description#description
The authors acknowledge Journal of Controlled Release (Elsevier) for allowing them to archive this paper.

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