Virulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets

[en] Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes for the heat-stable (STa and STb) and heat-labile (LT) enterotoxins, for the F4, F5, F6, and F41 fimbrial adhesion subunit, and also with five Rep probes for the RepFIA and RepFIB basic replicons, and the RepFIC family of basic replicons. With the exception of the F41 probe, the other virulence probes hybridized with at least one plasmid band of a size range from 65 to more than 100 Mda. Common associations of virulence factor-encoding genes on plasmid bands were: STb/LT, STa/F5, STa/F6, STa/STb. Other associations, STa/F4, STa/F4/F6, and STa/STb/LT/F6, were rarer. On the other hand the F4 adhesin-encoding genes were isolated on one plasmid band in all but three F4+ isolates. All but one of the 92 virulence plasmids which were studied have Rep probe hybridization profiles and replicon types typical of the uni- or multireplicon plasmids belonging to the various incompatibility groups of the F incompatibility complex.

Disciplines :

Microbiology

Author, co-author :

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires

Jacquemin, Etienne; Université de Liège - ULiège - ULG > Maladies infectieuses et parasitaires > Bactériologie et Pathologie des Maladies Bactériennes

Pohl, Pohl; INRV

Kaeckenbeeck, Albert ; Université de Liège - ULiège > Maladies infectieuses et parasitaires > Bactériologie et Pathologie des Maladies Bactériennes

Language :

English

Title :

Virulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets

Publication date :

1998

Journal title :

Veterinary Microbiology

ISSN :

0378-1135

eISSN :

1873-2542

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

291-301

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 14 September 2008

Statistics

Number of views

134 (4 by ULiège)

Number of downloads

257 (3 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Alexander, T.J.L., 1994. Neonatal Diarrhoea in Pigs. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB International, Guildford, UK, pp. 151-170.
Bergquist P.L., Saadi S., Maas W.K. Distribution of basic replicons having homology with RepFIA, RTepFIB and RepFIC among incF group plasmids. Plasmid. 15:1986;19-34.
Bertschinger, H.U., Gyles, C.L., 1994. Oedema Disease of Pigs. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB International, Guildford, UK, pp. 193-220.
Broes A., Fairbrother J.M., Mainil J.G., Harel J., Larivière S. Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli serotypes O8:KX105 and O8:K2829 strains isolated from piglets with diarrhea. J. Clin. Microbiol. 26:1988;2402-2409.
Casey T.A., Moon H.W. Genetic characterization and virulence of enterotoxigenic Escherichia coli mutants which have lost virulence genes in vivo. Infect. Immun. 58:1990;4156-4158.
Chaslus-Dancla E., Pohl P., Meurisse M., Marin M., Lafont J.P. High genetic homology between plasmids of human and animal origins conferring resistance to the aminoglycosides gentamycin and apramycin. Antimicrob. Agents Chemother. 35:1991;590-593.
Couturier M., Bex F., Bergquist P.L., Maas W.K. Identification and classification of bacterial plasmids. Microbiol. Rev. 52:1988;305-317.
Fairbrother P. Les colibacilloses du porc. Ann. Méd. Vét. 137:1993;369-375.
Fairbrother, J.M., Ngeleka, M., 1994. Extraintestinal Escherichia coli infections in pigs. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans, CAB International, Guildford, UK, pp. 221-236.
Gyles, C.L., 1994. Escherichia coli enterotoxins. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans, CAB International, Guildford, UK, pp. 337-364.
Hampson, D.J., 1994. Postweaning Escherichia coli diarrhoea in pigs. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans, CAB International, Guildford, UK, pp. 171-192.
Harnett N.M., Gyles C.L. Enterotoxigenicity of bovine and porcine Escherichia coli of O groups 8, 9, 20, 64, 101, and X46. Am. J. Vet. Res. 44:1983;1210-1214.
Harnett N.M., Gyles C.L. Linkage of genes for heat-stable enterotoxin, drug resistance, K99 antigen, and colicin production in bovine and porcine strains of enterotoxigenic Escherichia coli. Am. J. Vet. Res. 46:1985;428-433.
Holland R.E. Some infectious causes of diarrhea in young farm animals. Clin. Microbiol. Rev. 3:1990;345-375.
Isaacson, R.E., Schneider, R.A., Moon, H.W., 1978. Escherichia coli in the small intestines of calves with and without diarrhea: identification of virulence factors. In: Acres, S.D. (Ed.), Proceedings of the Second International Symposium on Neonatal Diarrhea, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, pp. 17-28.
Kado C.I., Liu S.T. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145:1981;1365-1373.
Lior, H., 1994. Classification of Escherichia coli. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB International, Guildford, UK, pp. 31-72.
Maas R., Silva A.M., Gomes T.A.T., Trabulsi L.R., Maas W.K. Detection of genes for heat-stable enterotoxin I in Escherichia coli strains isolated in Brazil. Infect. Immun. 49:1985;46-51.
Mainil J.G., Bex F., Dreze P., Pohl P., Kaeckenbeeck A., Couturier M. Replicon typing of virulence plasmids in enterotoxigenic Escherichia coli isolates from cattle. Infect. Immun. 60:1992;3376-3380.
Mainil J.G., Bex F., Jacquemin E., Pohl P.H., Couturier M., Kaeckenbeeck A. Prevalence of four enterotoxin (STaP, STaH, STb, and LT) and four adhesin subunit (K99, K88, 987P, and F41) genes among Escherichia coli isolates from cattle. Am. J. Vet. Res. 51:1990;187-190.
Mainil J.G., Daube G., Jacquemin E., Kaeckenbeeck A., Pohl P.H. Typage des Escherichia coli isolées d'intestins de porcelets en Belgique au moyen de sondes génétiques: souches entérotoxinogènes, vérotoxinogènes ou entéropathogènes. Ann. Méd. Vét. 139:1995;5-13.
Mainil J.G., Sadowski P.L., Tarsio M., Moon H.W. In vivo emergence of enterotoxigenic Escherichia coli variants lacking genes for K99 fimbriae and heat-stable enterotoxin. Infect. Immun. 55:1987;3111-3116.
Moon H.W., Nagy B., Isaacson R.E., Orskov I. Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic Escherichia coli from calves and pigs. Infect. Immun. 15:1977;614-620.
Nagy B., Moon H.W., Isaacson R.E. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic Escherichia coli. Infect. Immun. 16:1977;344-352.
O'Brien A.D., Holmes R.K. Shiga and Shiga-like toxins. Microbiol. Rev. 51:1987;206-220.
Oswald E., De Rycke J., Guillot J.F., Boivin R. Cytotoxic effect of multinucleation in HeLa cell cultures associated with the presence of Vir plasmid in Escherichia coli. FEMS Microbiol. Letters. 58:1989;95-100.
Pohl P. Les souches pathogènes d'Escherichia coli: histoire et classification. Ann. Méd. Vét. 137:1993;325-333.
Pohl P., Lintermans P., Bex F., Desmyter A., Dreze P., Fonteyne P.A., Couturier M. Propriétés phénotypiques et génotypiques de quatre plasmides de virulence de Salmonella typhimurium. Ann. Inst. Pasteur (Paris). 138:1987;523-528.
Silva R.M., Saadi S., Maas W.K. A basic replicon of virulence-associated plasmids of Shigella sp. and enteroinvasive Escherichia coli is homologous with a basic replicon in plasmids of incF groups. Infect. Immun. 56:1988;836-842.
Smith, H.R., Scotland, S.M., Rowe, B., 1985. Genetics of Escherichia coli Virulence. In: Sussman, M. (Ed.), The virulence of Escherichia coli. The Society for General Microbiology, Academic Press, London, UK, pp. 227-269.
Smyth, C.J., Marron, M., Smith, S.G.J., 1994. Fimbriae of Escherichia coli. In: Gyles, C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB International, Guildford, UK, pp. 399-436.
So M., Heffron F., McCarthy B.J. The Escherichia coli gene encoding heat-stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 277:1979;453-456.
Sojka W.J. Colibacillose intestinale des porcelets. Ann. Méd. Vét. 116:1972;377-446.
Thiry G. Plasmids of the epiphytic bacterium Erwinia uredovora. J. Gen. Microbiol. 130:1984;1623-1631.
Tinger S.A., Curtiss III R. Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons. J. Bacteriol. 130:1990;1623-1631.
Whipp S.C., Moon H.W., Argenzio R.A. Comparison of enterotoxic activities of heat-stable enterotoxins from class 1 and class 2 Escherichia coli of swine origin. Infect. Immun. 31:1981;245-251.