Reference : RhoA inhibition is a key step in pituicyte stellation induced by A(1)-type adenosine ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/5417
RhoA inhibition is a key step in pituicyte stellation induced by A(1)-type adenosine receptor activation.
English
Rosso, Lia [>Université de Nice-Sophia Antipolis, France > > >CNRS-UMR 6548 > Laboratoire de physiologie Cellulaire et Moléculaire > >]
Peteri-Brunback, Brigitta [>Université de Nice-Sophia Antipolis, France > > >CNRS-UMR 6548 > Laboratoire de physiologie Cellulaire et Moléculaire > > > > > >]
Vouret-Craviari, Valerie [Université de Nice-Sophia-Antipolis, France > > >Centre Antoine Lacassagne > > >]
Deroanne, Christophe mailto [Université de Nice-Sophia-Antipolis, France > > >Centre Antoine Lacassagne > > > >]
Troadec, Jean-Denis [>Université de Nice-Sophia Antipolis, France > > >CNRS-UMR 6548 > Laboratoire de physiologie Cellulaire et Moléculaire > > > > > >]
Thirion, Sylvie [>Université de Nice-Sophia Antipolis, France > > >CNRS-UMR 6548 > Laboratoire de physiologie Cellulaire et Moléculaire > > > > > >]
Van Obberghen-Schilling, Ellen [>Université de Nice-Sophia-Antipolis, France > > >Centre Antoine Lacassagne > > > > > >]
Mienville, Jean-Marc [>Université de Nice-Sophia Antipolis, France > > >CNRS-UMR 6548 > Laboratoire de physiologie Cellulaire et Moléculaire > > > > > >]
2002
Glia
Wiley Liss, Inc.
38
4
351-62
Yes (verified by ORBi)
International
0894-1491
1098-1136
New York
NY
[en] Adenosine/metabolism/pharmacology ; Adenosine Triphosphatases/antagonists & inhibitors/metabolism ; Adenosine Triphosphate/analogs & derivatives/metabolism/pharmacology ; Animals ; Cell Size/physiology ; Cells, Cultured ; Cytoskeleton/drug effects/metabolism ; Enzyme Inhibitors/pharmacology ; Feedback/drug effects/physiology ; Lysophospholipids/pharmacology ; MAP Kinase Signaling System/drug effects/physiology ; Oxytocin/metabolism/pharmacology ; Pituitary Gland, Posterior/cytology/enzymology/secretion ; Rats ; Rats, Wistar ; Receptors, Purinergic P1/drug effects/metabolism ; Signal Transduction/drug effects/physiology ; Vasopressins/metabolism/pharmacology ; rhoA GTP-Binding Protein/agonists/antagonists & inhibitors/metabolism
[en] Pituicyte stellation in vitro represents a useful model with which to study morphological changes that occur in vivo in these cells during times of high neurohypophysial hormone output. This model has helped us establish the hypothesis of a purinergic regulation of pituicyte morphological plasticity. We first show that ATP induces stellation in 37% of pituicytes, an effect that is secondary to the metabolism of ATP to adenosine. Adenosine-induced stellation of pituicytes appears to be mediated by A(1)-type receptors. The effect is independent of intracellular calcium and does not involve the mitogen-activated protein kinase pathway. The basal (nonstellate) state of pituicytes depends on tonic activation of a Rho GTPase because both C3 transferase (a Rho inhibitor) and Y-27632 (an inhibitor of p160Rho kinase) can induce stellation. Lysophosphatidic acid, a Rho activator, blocks the morphogenic effect of adenosine dose-dependently. Using a specific RhoA pull-down assay, we also show that downregulation of activated RhoA is the key event coupling A(1) receptor activation to pituicyte stellation, via F-actin depolymerization and microtubule reorganization. Finally, both vasopressin and oxytocin can prevent or reverse adenosine-induced stellation. The effects of vasopressin, and those of high concentrations of oxytocin, are mediated through V(1a) receptors. Placed within the context of the relevant literature, our data suggest the possibility of a purinergic regulation of pituicyte morphological plasticity and subsequent modulation of hormone release, with these hormones providing a negative feedback mechanism.
Researchers
http://hdl.handle.net/2268/5417
10.1002/glia.10072
Copyright 2002 Wiley-Liss, Inc.

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